Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering

• Published in: The Plant journal : for cell and molecular biology Vol. 55; no. 5; pp. 857 - 868
• Main Authors: Hasunuma, Tomohisa, Miyazawa, Shin‐Ichi, Yoshimura, Satomi, Shinzaki, Yuki, Tomizawa, Ken‐Ichi, Shindo, Kazutoshi, Choi, Seon‐Kang, Misawa, Norihiko, Miyake, Chikahiro
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2008; Blackwell Science
• Subjects: aerial parts; astaxanthin; Bacteria; beta-carotene; Biological and medical sciences; Biosynthesis; Botany; Brevundimonas; Carbon dioxide; carotenoid; Caulobacteraceae; Caulobacteraceae - genetics; chloroplasts; color; developmental stages; DNA, Plant; DNA, Plant - genetics; engineering; Fundamental and applied biological sciences. Psychology; Gene expression; gene transfer; genes; Genes, Bacterial; Genetic Engineering; genetics; Genome, Chloroplast; human health; Leaves; Light intensity; market prices; metabolic engineering; Metabolism; Metabolism. Physicochemical requirements; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana tabacum; Nitrogen; Nitrogen - metabolism; Oxygenases; Oxygenases - genetics; photons; Photosynthesis; Plant Leaves; Plant Leaves - genetics; Plant Leaves - metabolism; Plant physiology and development; Plants, Genetically Modified; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; plastid transformation; Plastids; Plastids - genetics; Ribulose-Bisphosphate Carboxylase; Ribulose-Bisphosphate Carboxylase - metabolism; RNA, Plant; RNA, Plant - genetics; Tobacco; Transgenic plants; Xanthophylls; Xanthophylls - biosynthesis; Enzyme; Growth; Hydroxylase; Carbon dioxide; Biosynthesis; Plant leaf; Plastid; Nicotiana tabacum; Genetic transfer; astaxanthin; plastid transformation; Gene; Dicotyledones; Angiospermae; Above ground plant part; Spermatophyta; Genetic engineering; Transgenic plant; Oxidoreductases; Solanaceae; metabolic engineering; Photosynthesis; Carotenoid; Chloroplast

Summary The natural pigment astaxanthin has attracted much attention because of its beneficial effects on human health, despite its expensive market price. In order to produce astaxanthin, transgenic plants have so far been generated through conventional genetic engineering of Agrobacterium‐mediated...