Novel lycopene epsilon cyclase activities in maize revealed through perturbation of carotenoid biosynthesis

• Published in: The Plant journal : for cell and molecular biology Vol. 59; no. 4; pp. 588 - 599
• Main Authors: Bai, Ling, Kim, Eun‐Ha, DellaPenna, Dean, Brutnell, Thomas P.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.2009; Blackwell
• Subjects: Accumulation; Alleles; Amino acids; Biochemistry; Biological and medical sciences; Biosynthesis; Botany; breeding; Carotenoids; Carotenoids - biosynthesis; Corn; E coli; embryo; embryology; Embryos; endosperm; enzymes; enzymology; epsilon‐carotene; Escherichia coli; Fundamental and applied biological sciences. Psychology; Gene expression; gene expression regulation; Gene Expression Regulation, Plant; genes; genetics; humans; Intramolecular Lyases; Intramolecular Lyases - genetics; Intramolecular Lyases - metabolism; lycopene; lycopene beta‐cyclase; metabolism; Molecular and cellular biology; Molecular genetics; Mutagenesis, Site-Directed; mutants; Mutation; Plant Proteins; Plant Proteins - genetics; Plant Proteins - metabolism; RNA, Plant; RNA, Plant - genetics; Seeds; Seeds - enzymology; Seeds - genetics; site-directed mutagenesis; Substrate Specificity; Tissues; transcription (genetics); Transcription. Transcription factor. Splicing. Rna processing; transcriptional regulation; Zea mays; Zea mays - embryology; Zea mays - enzymology; Zea mays - genetics; epsilon-carotene; Embryo; Transcription; carotenoids; Carotene; transcriptional regulation; Biosynthesis; Perturbation; Lycopene; Chenopodiaceae; Beta; Regulation(control); lycopene beta-cyclase; Dicotyledones; Angiospermae; Spermatophyta; Endosperm; Carotenoid

Summary In maize, human selection for yellow endosperm has led to diversification of grain carotenoid content and composition. This variation has remained largely untapped in modern breeding programs that have focused nearly exclusively on yield gains. In this paper, we show that carotenoid accumula...