An image processing pipeline for electron cryo‐tomography in RELION‐5

• Published in: FEBS open bio Vol. 14; no. 11; pp. 1788 - 1804
• Main Authors: Burt, Alister, Toader, Bogdan, Warshamanage, Rangana, Kügelgen, Andriko, Pyle, Euan, Zivanov, Jasenko, Kimanius, Dari, Bharat, Tanmay A. M., Scheres, Sjors H. W.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2024; John Wiley and Sons Inc; Wiley
• Subjects: Automation; Bioinformatics; Cryoelectron Microscopy - methods; Electron Microscope Tomography - methods; Electron microscopes; Electron Microscopy; electron tomography; Image processing; Image Processing, Computer-Assisted - methods; Imaging, Three-Dimensional - methods; Metadata; Method; Microscopy; Models, Molecular; Open source software; Software; Structural Biology; Tomography; User interface; image processing; electron microscopy; software; electron tomography

Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three‐dimensional tomographic reconstructions, averaging images of multiple instances of...