Expression of the chemokine superfamily in rheumatoid arthritis

• Published in: Clinical and experimental immunology Vol. 97; no. 3; pp. 451 - 457
• Main Authors: HOSAKA, S., AKAHOSHI, T., WADA, C., KONDO, H.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.1994; Blackwell
• Subjects: Arthritis, Rheumatoid - immunology; Base Sequence; Biological and medical sciences; Cells, Cultured; chemokine superfamily; Chemotactic Factors - biosynthesis; Chemotactic Factors - genetics; Cytokines - biosynthesis; Cytokines - genetics; Diseases of the osteoarticular system; DNA Primers; Fibroblasts - immunology; Gene Expression; Humans; Immunoprecipitation; Inflammatory joint diseases; Medical sciences; Molecular Sequence Data; Oligonucleotide Probes; rheumatoid arthritis; RNA, Messenger - analysis; RNA-Directed DNA Polymerase; RT‐PCR; Synovial Membrane - cytology; Synovial Membrane - immunology; Human; Immunopathology; Leukocyte; Cytokine; Diseases of the osteoarticular system; Inflammatory joint disease; Gene expression; Chemotactic factor; Blood; Polymerase chain reaction; Synovial fluid; Immunoprecipitation reaction; Rheumatoid arthritis; Inflammatory cell; Fibroblast
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.1994.tb06109.x

SUMMARY The infiltration of leucocytes into the Joint of rheumatoid arthritis (RA) is believed to be mediated by chemotactic factors released by activated cells. In this study, examination was made of the gene expression and production of the chemokine superfamily in RA patients by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and immunoprecipitation. Cultured synovial fibroblasts were found capable of expressing and producing IL‐8, GRO, monocyte chemotactie and activating factor (MCAF), macrophage inflammatory protein‐la (MIP‐1α), MIP‐1β and RANTES in response to IL‐1α, The expression of IL‐8, GRO. MCAF, MIP‐1α, and MIP‐1β was clearly shown to increase in freshly isolated synovial fluid mononuclear cells (SFMC) of RA patients, in contrast to peripheral blood mononuclear cells (PBMC) of RA patients and normal subjects. The gene expression of RANTES appeared to be the same for RA SFMC. RA PBMC, and normal PBMC. Thus, the over‐expression of various chemokines may promote the recruitment of inflammatory cells into rheumatoid inflamed joints.