实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能
R512.6+3; 目的 观察慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMCs)中T细胞受体重排删除环(TRECs)的含量,从而推测CHC患者的胸腺近期输出功能,并且比较TRECs含量与HCV载量的关系.方法 利用实时荧光定量PCR方法检测22例CHC患者及16例正常人PBMCs中TRECs含量,用RT-PCR方法结合荧光探针的检测技术检测CHC组外周血血清中的HCVRNA.结果 CHC患者中TRECs含量为(5.52±1.87)copies/103PBMCs,低于正常人TRECs水平(7.19±2.42)copies/103PBMCs,P＜0.05;TRECs含量与HCV载量直线相关分...
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| Published in | 临床肝胆病杂志 Vol. 23; no. 6; pp. 432 - 433 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
徐州医学院附属医院感染病科,江苏,徐州,221002
2007
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-5256 |
| DOI | 10.3969/j.issn.1001-5256.2007.06.012 |
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| Abstract | R512.6+3; 目的 观察慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMCs)中T细胞受体重排删除环(TRECs)的含量,从而推测CHC患者的胸腺近期输出功能,并且比较TRECs含量与HCV载量的关系.方法 利用实时荧光定量PCR方法检测22例CHC患者及16例正常人PBMCs中TRECs含量,用RT-PCR方法结合荧光探针的检测技术检测CHC组外周血血清中的HCVRNA.结果 CHC患者中TRECs含量为(5.52±1.87)copies/103PBMCs,低于正常人TRECs水平(7.19±2.42)copies/103PBMCs,P＜0.05;TRECs含量与HCV载量直线相关分析r=-0.427,P＜0.05.结论 CHC患者TRECs水平低于正常人,显示其胸腺近期输出功能明显降低,且与HCV载量呈负相关性. |
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| AbstractList | R512.6+3; 目的 观察慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMCs)中T细胞受体重排删除环(TRECs)的含量,从而推测CHC患者的胸腺近期输出功能,并且比较TRECs含量与HCV载量的关系.方法 利用实时荧光定量PCR方法检测22例CHC患者及16例正常人PBMCs中TRECs含量,用RT-PCR方法结合荧光探针的检测技术检测CHC组外周血血清中的HCVRNA.结果 CHC患者中TRECs含量为(5.52±1.87)copies/103PBMCs,低于正常人TRECs水平(7.19±2.42)copies/103PBMCs,P＜0.05;TRECs含量与HCV载量直线相关分析r=-0.427,P＜0.05.结论 CHC患者TRECs水平低于正常人,显示其胸腺近期输出功能明显降低,且与HCV载量呈负相关性. |
| Author | 汪莉萍 葛国洪 张言超 陈明 郭海燕 |
| AuthorAffiliation | 徐州医学院附属医院感染病科,江苏,徐州,221002 |
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| Keywords | TRECs 实时荧光定量PCR 胸腺近期输出功能 慢性丙型肝炎 |
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| Title | 实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能 |
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