粒细胞-巨噬细胞集落刺激因子转染对白血病细胞K562形态及增殖的影响
目的观察重组PcDNA3.1-粒细胞-巨噬细胞集落刺激因子（GM-CSF）、外源性GM-CSF对髓系白血病细胞（K562）形态及增殖的影响。方法将K562细胞分为4组,重组质粒组、外源性组、空质粒组细胞分别以重组PcDNA3.1-GM-CSF、外源性GM-CSF、PcDNA3.1空质粒转染,细胞对照组细胞未转染任何质粒,观察各组细胞形态学变化及细胞增殖情况。结果光学显微镜下,细胞对照组、空质粒组及外源性组细胞形态相似,细胞着色呈深蓝色,细胞质和细胞核不易区分;重组质粒组细胞形态和其余3组明显不同,细胞质呈浅蓝色,细胞核呈淡粉红色,分界清楚,部分细胞内可见肾形、马蹄形细胞核。显微镜油镜下,细胞对...
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| Published in | 山东医药 Vol. 57; no. 31; pp. 5 - 8 |
|---|---|
| Main Author | |
| Format | Magazine Article |
| Language | Chinese |
| Published |
川北医学院医学微生物学与免疫学教研室,四川南充,637000
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-266X |
| DOI | 10.3969/j.issn.1002-266X.2017.31.002 |
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| Abstract | 目的观察重组PcDNA3.1-粒细胞-巨噬细胞集落刺激因子（GM-CSF）、外源性GM-CSF对髓系白血病细胞（K562）形态及增殖的影响。方法将K562细胞分为4组,重组质粒组、外源性组、空质粒组细胞分别以重组PcDNA3.1-GM-CSF、外源性GM-CSF、PcDNA3.1空质粒转染,细胞对照组细胞未转染任何质粒,观察各组细胞形态学变化及细胞增殖情况。结果光学显微镜下,细胞对照组、空质粒组及外源性组细胞形态相似,细胞着色呈深蓝色,细胞质和细胞核不易区分;重组质粒组细胞形态和其余3组明显不同,细胞质呈浅蓝色,细胞核呈淡粉红色,分界清楚,部分细胞内可见肾形、马蹄形细胞核。显微镜油镜下,细胞对照组、外源性组及空质粒组结果相似,细胞着色深,呈深蓝色,细胞内未见任何颗粒沉积;重组质粒组部分细胞着色呈深蓝色,部分细胞着色呈浅粉色,呈浅粉色的部分细胞内可见有斑点状甲臜颗粒沉积。与其余2组比较,重组质粒组第3、5、7、9天细胞增殖抑制率升高（P均〈0.05）;与空质粒组比较,外源性组第3、5、7天细胞增殖抑制率升高（P均〈0.05）。结论重组PcDNA3.1-GM-CSF及外源性GM-CSF均能使K562细胞形态发生改变、增殖受到抑制,作用强度随作用时间延长而增加,且重组PcDNA3.1-GM-CSF抑制作用强于外源性GM-CSF。 |
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| AbstractList | R730.5; 目的 观察重组PcDNA3.1-粒细胞-巨噬细胞集落刺激因子(GM-CSF)、外源性GM-CSF对髓系白血病细胞(K562)形态及增殖的影响.方法 将K562细胞分为4组,重组质粒组、外源性组、空质粒组细胞分别以重组PcDNA3.1-GM-CSF、外源性GM-CSF、PcDNA3.1空质粒转染,细胞对照组细胞未转染任何质粒,观察各组细胞形态学变化及细胞增殖情况.结果 光学显微镜下,细胞对照组、空质粒组及外源性组细胞形态相似,细胞着色呈深蓝色,细胞质和细胞核不易区分;重组质粒组细胞形态和其余3组明显不同,细胞质呈浅蓝色,细胞核呈淡粉红色,分界清楚,部分细胞内可见肾形、马蹄形细胞核.显微镜油镜下,细胞对照组、外源性组及空质粒组结果相似,细胞着色深,呈深蓝色,细胞内未见任何颗粒沉积;重组质粒组部分细胞着色呈深蓝色,部分细胞着色呈浅粉色,呈浅粉色的部分细胞内可见有斑点状甲臜颗粒沉积.与其余2组比较,重组质粒组第3、5、7、9天细胞增殖抑制率升高(P均<0.05);与空质粒组比较,外源性组第3、5、7天细胞增殖抑制率升高(P均<0.05).结论 重组PcDNA3.1-GM-CSF及外源性GM-CSF均能使K562细胞形态发生改变、增殖受到抑制,作用强度随作用时间延长而增加,且重组PcDNA3.1-GM-CSF抑制作用强于外源性GM-CSF. 目的观察重组PcDNA3.1-粒细胞-巨噬细胞集落刺激因子（GM-CSF）、外源性GM-CSF对髓系白血病细胞（K562）形态及增殖的影响。方法将K562细胞分为4组,重组质粒组、外源性组、空质粒组细胞分别以重组PcDNA3.1-GM-CSF、外源性GM-CSF、PcDNA3.1空质粒转染,细胞对照组细胞未转染任何质粒,观察各组细胞形态学变化及细胞增殖情况。结果光学显微镜下,细胞对照组、空质粒组及外源性组细胞形态相似,细胞着色呈深蓝色,细胞质和细胞核不易区分;重组质粒组细胞形态和其余3组明显不同,细胞质呈浅蓝色,细胞核呈淡粉红色,分界清楚,部分细胞内可见肾形、马蹄形细胞核。显微镜油镜下,细胞对照组、外源性组及空质粒组结果相似,细胞着色深,呈深蓝色,细胞内未见任何颗粒沉积;重组质粒组部分细胞着色呈深蓝色,部分细胞着色呈浅粉色,呈浅粉色的部分细胞内可见有斑点状甲臜颗粒沉积。与其余2组比较,重组质粒组第3、5、7、9天细胞增殖抑制率升高（P均〈0.05）;与空质粒组比较,外源性组第3、5、7天细胞增殖抑制率升高（P均〈0.05）。结论重组PcDNA3.1-GM-CSF及外源性GM-CSF均能使K562细胞形态发生改变、增殖受到抑制,作用强度随作用时间延长而增加,且重组PcDNA3.1-GM-CSF抑制作用强于外源性GM-CSF。 |
| Abstract_FL | Objective To observe the effects of exogenous granulocyte-macrophage colony-stimulating factor (GM-CSF) and recombinant PcDNA3.1-GM-CSF on the morphology and proliferation of myeloid leukemia cells K562.Methods K562 cells were randomly divided into four groups.Recombinant PcDNA3.1-GM-CSF, exogenous GM-CSF, and PcDNA3.1 null plasmids were transfected into K562 cells as the recombinant plasmid group, the exogenous group and the empty plasmid group, respectively, and the non-transfected plasmid was set as the control group.The morphological changes of cells were observed under the optical microscope by using high power lens and oil lens, respectively, and the cell proliferation was measured by MTT.Results Morphological changes seen under high power lens: the control group, the empty plasmid group and exogenous group were similar in morphology, cells were stained dark blue, whereas no clear boundary was seen between the cytoplasm and nucleus;compared with these three groups, the cells of the recombinant plasmid group showed a significant difference, the cytoplasm was stained light blue, and the nuclei were pale pink along with a clear bounds between them.Kidney or horseshoe shaped nuclei could be seen in some of the cells.Morphological changes seen under oil lens: the control group, the exogenous group, and empty plasmid group were similar, the cells were stained dark blue, and granules deposition wasn''''t seen in the cells;as to the recombinant plasmid group, some cells were stained dark blue, while some were pale pink in which dotted formazan particles were seen.Results of cell proliferation assay: Compared with the other two groups, the inhibition rate of cell proliferation in the recombinant plasmid group showed an increase on the 3th , 5th, 7th and 9th days (P<0.05).Compared with the empty plasmid group, the inhibition rate in the exogeneous group displayed an increase on the 3th, 5th and 7th days (P<0.05).Conclusion Both recombinant PcDNA3.1-GM-CSF and exogenous GM-CSF could induce morphological change and lead to proliferation inhibition in K562 cells, the effects increase along with time, and the inhibitory effect of recombinant PcDNA3.1-GM-CSF is stronger than that of the exogenous GM-CSF. |
| Author | 陈蓉 常晋霞 李雪璐 彭丽娟 朱红 |
| AuthorAffiliation | 川北医学院医学微生物学与免疫学教研室,四川南充637000 |
| AuthorAffiliation_xml | – name: 川北医学院医学微生物学与免疫学教研室,四川南充,637000 |
| Author_FL | CHANG Jinxia ZHU Hong LI Xuelu PENG Lijuan CHEN Rong |
| Author_FL_xml | – sequence: 1 fullname: CHEN Rong – sequence: 2 fullname: CHANG Jinxia – sequence: 3 fullname: LI Xuelu – sequence: 4 fullname: PENG Lijuan – sequence: 5 fullname: ZHU Hong |
| Author_xml | – sequence: 1 fullname: 陈蓉 常晋霞 李雪璐 彭丽娟 朱红 |
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| Keywords | 粒细胞-巨噬细胞集落刺激因子 cell proliferation 细胞形态 granulocyte-macrophage colony-stimulating factor 白血病细胞 leukemic cells cell morphology 细胞增殖 |
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| Notes | granulocyte-macrophage colony-stimulating factor; leukemic cells; cell morphology; cell proliferation 37-1156/R Objective To observe the effects of exogenous granulocyte-macrophage colony-stimulating factor（GMCSF） and recombinant PcDNA3.1-GM-CSF on the morphology and proliferation of myeloid leukemia cells K562.Methods K562 cells were randomly divided into four groups.Recombinant PcDNA3.1-GM-CSF,exogenous GM-CSF,and PcDNA3.1 null plasmids were transfected into K562 cells as the recombinant plasmid group,the exogenous group and the empty plasmid group,respectively,and the non-transfected plasmid was set as the control group.The morphological changes of cells were observed under the optical microscope by using high power lens and oil lens,respectively,and the cell proliferation was measured by MTT.Results Morphological changes seen under high power lens：the control group,the empty plasmid group and exogenous group were similar in morphology,cells were stained dark blue,whereas no clear boundary was seen between t |
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| Snippet | ... R730.5; 目的 观察重组PcDNA3.1-粒细胞-巨噬细胞集落刺激因子(GM-CSF)、外源性GM-CSF对髓系白血病细胞(K562)形态及增殖的影响.方法... |
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| SubjectTerms | 白血病细胞 粒细胞-巨噬细胞集落刺激因子 细胞增殖 细胞形态 |
| Title | 粒细胞-巨噬细胞集落刺激因子转染对白血病细胞K562形态及增殖的影响 |
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