Ultra-Sensitive TP53 Sequencing for Cancer Detection Reveals Progressive Clonal Selection in Normal Tissue over a Century of Human Lifespan

• Published in: Cell reports (Cambridge) Vol. 28; no. 1; pp. 132 - 144.e3
• Main Authors: Salk, Jesse J., Loubet-Senear, Kaitlyn, Maritschnegg, Elisabeth, Valentine, Charles C., Williams, Lindsey N., Higgins, Jacob E., Horvat, Reinhard, Vanderstichele, Adriaan, Nachmanson, Daniela, Baker, Kathryn T., Emond, Mary J., Loter, Emily, Tretiakova, Maria, Soussi, Thierry, Loeb, Lawrence A., Zeillinger, Robert, Speiser, Paul, Risques, Rosa Ana
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.07.2019; Elsevier
• Subjects: Adult; Aged; Aged, 80 and over; aging; Aging - genetics; Biochemistry, Molecular Biology; Cancer; Cell-Free Nucleic Acids - genetics; Clonal Evolution - genetics; Databases, Genetic; DNA, Neoplasm - genetics; Duplex Sequencing; early detection; Female; Genomics; gynecologic oncology; high-grade serous ovarian cancer; High-Throughput Nucleotide Sequencing; Humans; Infant, Newborn; Life Sciences; Middle Aged; Mutation; next-generation sequencing; Ovarian Neoplasms - diagnosis; Ovarian Neoplasms - genetics; Ovarian Neoplasms - pathology; Selection, Genetic; Sequence Analysis, DNA; somatic mutations; TP53, clonal evolution; Tumor Suppressor Protein p53 - genetics; uterine lavage; Uterus - metabolism; somatic mutations; uterine lavage; next-generation sequencing; high-grade serous ovarian cancer; TP53, clonal evolution; Duplex Sequencing; gynecologic oncology; early detection; aging; clonal evolution; TP53

High-accuracy next-generation DNA sequencing promises a paradigm shift in early cancer detection by enabling the identification of mutant cancer molecules in minimally invasive body fluid samples. We demonstrate 80% sensitivity for ovarian cancer detection using ultra-accurate Duplex Sequencing to i...