An unusual feature associated with LEE1 P1 promoters in enteropathogenic Escherichia coli (EPEC)

Summary Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing σ70, it is presumed that specific sequence in one or more of the −10, extended −10 and −35 elements of the promoter guides...

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Published in	Molecular microbiology Vol. 83; no. 3; pp. 612 - 622
Main Authors	Jeong, Jae-Ho, Kim, Hyun-Ju, Kim, Kun-Hee, Shin, Minsang, Hong, Yeongjin, Rhee, Joon Haeng, Schneider, Thomas D., Choy, Hyon E.
Format	Journal Article
Language	English
Published	Oxford, UK Blackwell Publishing Ltd 01.02.2012 Blackwell
Subjects	Bacteriology Base Sequence Biochemistry Biological and medical sciences Biosynthesis E coli Enteropathogenic Escherichia coli - genetics Escherichia coli Escherichia coli Proteins - genetics Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Microbiology Miscellaneous Molecular Sequence Data Operon Promoter Regions, Genetic RNA polymerase Software Trans-Activators - genetics Transcription Initiation Site Transcription, Genetic Bacteria Escherichia coli Enterobacteriaceae
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Abstract	Summary Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing σ70, it is presumed that specific sequence in one or more of the −10, extended −10 and −35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic E. coli and found two promoters separated by 10 bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighbouring As at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF.
AbstractList	Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing σ70, it is presumed that specific sequence in one or more of the −10, extended −10 and −35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic Escherichia coli (EPEC) and found two promoters separated by 10 bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighboring A s at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF. Summary Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing σ70, it is presumed that specific sequence in one or more of the −10, extended −10 and −35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic E. coli and found two promoters separated by 10 bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighbouring As at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF. Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing σ70, it is presumed that specific sequence in one or more of the -10, extended -10 and -35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic E. coli and found two promoters separated by 10 bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighbouring As at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF. Transcription start points in bacteria are influenced by the nature of the RNA polymerase-promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing ...70, it is presumed that specific sequence in one or more of the -10, extended -10 and -35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic E. coli and found two promoters separated by 10 bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighbouring As at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF. (ProQuest: ... denotes formulae/symbols omitted.) Summary Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing σ70, it is presumed that specific sequence in one or more of the −10, extended −10 and −35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic E. coli and found two promoters separated by 10 bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighbouring As at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF. Transcription start points in bacteria are influenced by the nature of the RNA polymerase.promoter interaction. For Escherichia coli RNA polymerase holoenzyme containing sigma 70, it is presumed that specific sequence in one or more of the -10, extended -10 and -35 elements of the promoter guides the RNAP to select the cognate start point. Here, we investigated the promoter driving expression of the LEE1 operon in enteropathogenic E.coli and found two promoters separated by 10bp, LEE1 P1A (+1) and LEE1 P1B (+10) using various in vitro biochemical tools. A unique feature of P1B was the presence of multiple transcription starts from five neighbouring As at the initial transcribed region. The multiple products did not arise from stuttering synthesis. Analytical software based on information theory was employed to determine promoter elements. The concentration of the NTP pool altered the preferred transcription start points, albeit the underlying mechanism is elusive. Under in vivo conditions, dominant P1B, but not P1A, was subject to regulation by IHF.
Author	Shin, Minsang Rhee, Joon Haeng Kim, Kun-Hee Schneider, Thomas D. Jeong, Jae-Ho Hong, Yeongjin Kim, Hyun-Ju Choy, Hyon E.
AuthorAffiliation	1 Center for Host Defense against Enteropathogenic Bacteria Infection, Chonnam National University Medical School, Kwangju 501-746, South Korea 3 National Cancer Institute, National Institutes of Health, Gene Regulation and Chromosome Biology Laboratory, Building 539, Room 129A, NCI-Frederick, Maryland, United States 2 Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, South Korea
AuthorAffiliation_xml	– name: 2 Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, South Korea – name: 3 National Cancer Institute, National Institutes of Health, Gene Regulation and Chromosome Biology Laboratory, Building 539, Room 129A, NCI-Frederick, Maryland, United States – name: 1 Center for Host Defense against Enteropathogenic Bacteria Infection, Chonnam National University Medical School, Kwangju 501-746, South Korea
Author_xml	– sequence: 1 givenname: Jae-Ho surname: Jeong fullname: Jeong, Jae-Ho organization: Center for Host Defense against Enteropathogenic Bacteria Infection – sequence: 2 givenname: Hyun-Ju surname: Kim fullname: Kim, Hyun-Ju organization: Center for Host Defense against Enteropathogenic Bacteria Infection – sequence: 3 givenname: Kun-Hee surname: Kim fullname: Kim, Kun-Hee organization: Center for Host Defense against Enteropathogenic Bacteria Infection – sequence: 4 givenname: Minsang surname: Shin fullname: Shin, Minsang organization: Center for Host Defense against Enteropathogenic Bacteria Infection – sequence: 5 givenname: Yeongjin surname: Hong fullname: Hong, Yeongjin organization: Center for Host Defense against Enteropathogenic Bacteria Infection – sequence: 6 givenname: Joon Haeng surname: Rhee fullname: Rhee, Joon Haeng organization: Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, South Korea – sequence: 7 givenname: Thomas D. surname: Schneider fullname: Schneider, Thomas D. organization: National Cancer Institute, National Institutes of Health, Gene Regulation and Chromosome Biology Laboratory, Building 539, Room 129A, NCI-Frederick, Maryland, USA – sequence: 8 givenname: Hyon E. surname: Choy fullname: Choy, Hyon E. email: hyonchoy@jnu.ac.kr organization: Center for Host Defense against Enteropathogenic Bacteria Infection
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Snippet	Summary Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase... Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme... Summary Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase... Transcription start points in bacteria are influenced by the nature of the RNA polymerase-promoter interaction. For Escherichia coli RNA polymerase holoenzyme... Transcription start points in bacteria are influenced by the nature of the RNA polymerase.promoter interaction. For Escherichia coli RNA polymerase holoenzyme... Transcription start points in bacteria are influenced by the nature of the RNA polymerase·promoter interaction. For Escherichia coli RNA polymerase holoenzyme...
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SubjectTerms	Bacteriology Base Sequence Biochemistry Biological and medical sciences Biosynthesis E coli Enteropathogenic Escherichia coli - genetics Escherichia coli Escherichia coli Proteins - genetics Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Microbiology Miscellaneous Molecular Sequence Data Operon Promoter Regions, Genetic RNA polymerase Software Trans-Activators - genetics Transcription Initiation Site Transcription, Genetic
Title	An unusual feature associated with LEE1 P1 promoters in enteropathogenic Escherichia coli (EPEC)
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