Reduced histone biosynthesis and chromatin changes arising from a damage signal at telomeres

• Published in: Nature structural & molecular biology Vol. 17; no. 10; pp. 1218 - 1225
• Main Authors: O'Sullivan, Roderick J, Kubicek, Stefan, Schreiber, Stuart L, Karlseder, Jan
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.10.2010; Nature Publishing Group
• Subjects: 631/337/100/102; 631/337/103/560; 631/337/1427/2566; 631/337/641; Aging; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Biosynthesis; Bleomycin - toxicity; Cell cycle; Cell Line; Cellular biology; Cellular Senescence - physiology; Chromatin; Chromatin - metabolism; Chromatin Immunoprecipitation; Deoxyribonucleic acid; DNA; DNA Damage; DNA Replication; Epigenesis, Genetic; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Genetic aspects; Histones; Histones - biosynthesis; Humans; Life Sciences; Membrane Biology; Methylation; Molecular biology; mRNA Cleavage and Polyadenylation Factors - biosynthesis; mRNA Cleavage and Polyadenylation Factors - genetics; Nuclear Proteins - biosynthesis; Nuclear Proteins - genetics; Oncogene Proteins, Viral - physiology; Papillomavirus E7 Proteins - physiology; Physiological aspects; Protein Processing, Post-Translational; Protein Structure; Repressor Proteins - physiology; Retinoblastoma Protein - physiology; Signal transduction; Telomere - physiology; Telomeres; Tumor Suppressor Protein p53 - physiology; United States

Telomere shortening, senescence and aging are connected, but how the signal at shortening telomeres is transmitted to the cell more globally is unclear. H3 and H4 synthesis is now shown to be reduced as cell cultures age. This alters expression of Asf1, a histone chaperone, compromising the ability...