Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays

• Published in: Analytical and bioanalytical chemistry Vol. 398; no. 7-8; pp. 3023 - 3032
• Main Authors: Heus, Ferry, Giera, Martin, de Kloe, Gerdien E, van Iperen, Dick, Buijs, Joost, Nahar, Tariq T, Smit, August B, Lingeman, Henk, de Esch, Iwan J. P, Niessen, Wilfried M. A, Irth, Hubertus, Kool, Jeroen
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.12.2010; Springer-Verlag; Springer
• Subjects: Acetylcholine binding protein; Analysis; Analytical Chemistry; Applied sciences; bioassays; Biochemistry; Biological Assay - instrumentation; Biological Assay - methods; Carrier Proteins - analysis; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - methods; Confocal; Exact sciences and technology; Flow Injection Analysis - methods; Fluorescence; Food Science; Global environmental pollution; Joining; Laboratory Medicine; LED-based confocal fluorescence detection; Liquid chromatography; Microfluidics; Microfluidics - instrumentation; Microfluidics - methods; Microscopy, Fluorescence - methods; Miniaturization; Monitoring/Environmental Analysis; Nanocomposites; Nanomaterials; Nanostructure; On-line systems; Original Paper; Other chromatographic methods; Pollution; Protein binding; Receptors; Tracers (Biology); Netherlands; Bioassay; Miniaturization; LED-based confocal fluorescence detection; Acetylcholine binding protein; Microfluidics; On line; Ligand; Mixture; Binding protein; Coupling; Flow injection; Fluid mechanics; Fluorescence detector; Biochemical analysis; Coupled method; Sample; Use; Biological indicator; Minimization; Liquid chromatography; Extracellular; Light emitting diode; Plate; Screening; Sensitivity; Volume; Analog; Reagents; Affinity; Acetylcholine; Detection; Tracers

One way to profile complex mixtures for receptor affinity is to couple liquid chromatography (LC) on-line to biochemical detection (BCD). A drawback of this hyphenated screening approach is the relatively high consumption of sample, receptor protein and (fluorescently labeled) tracer ligand. Here, w...