Engineered knottin peptide enables noninvasive optical imaging of intracranial medulloblastoma

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 110; no. 36; pp. 14598 - 14603
• Main Authors: Moore, Sarah J., Gephart, Melanie G. Hayden, Bergen, Jamie M., Su, YouRong S., Rayburn, Helen, Scott, Matthew P., Cochran, Jennifer R.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 03.09.2013; National Acad Sciences
• Subjects: Animals; antibodies; Biological Sciences; brain; Brain cancer; Brain neoplasms; Brain tumors; Cancer; caudal vein; Central nervous system; Cerebellar Neoplasms - diagnosis; Cerebellar Neoplasms - genetics; Cerebellar Neoplasms - metabolism; Chemotherapy; cystine; Cystine-Knot Miniproteins - chemistry; Cystine-Knot Miniproteins - genetics; Cystine-Knot Miniproteins - metabolism; Diagnostic Imaging - methods; drug therapy; Dyes; Female; fluorescence; fluorescent dyes; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; image analysis; Imaging; Injection; Integrin alpha5beta1 - metabolism; Integrins; Male; Medulloblastoma; Medulloblastoma - diagnosis; Medulloblastoma - genetics; Medulloblastoma - metabolism; Mice; Mice, Knockout; Mice, Nude; Mice, Transgenic; Microscopy, Fluorescence; Molecular imaging; Molecular Imaging - methods; Molecules; neoplasms; Nervous system; Neuroimaging; Patched Receptors; Peptides; Protein Binding; Protein Engineering; receptors; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; resection; Rodents; Sensitivity and Specificity; Tissues; Tumors; protein engineering; tumor targeting; miniprotein; Hedgehog pathway

Central nervous system tumors carry grave clinical prognoses due to limited effectiveness of surgical resection, radiation, and chemotherapy. Thus, improved strategies for brain tumor visualization and targeted treatment are critically needed. We demonstrate that mouse cerebellar medulloblastoma (MB...