Sex Identification Using the ZFX and ZFY Genes in Common Marmosets (Callithrix jacchus)

We investigated sex determination via the ZFX and ZFY genes using PCR-RFLP in the common marmoset. We designed a novel primer set to detect ZFX and ZFY. A 483-bp band from the ZFX gene and a 471-bp band from the ZFY gene were amplified. Sequencing data of the products amplified from ZFX and ZFY show...

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Published inExperimental Animals Vol. 60; no. 4; pp. 417 - 420
Main Authors TAKABAYASHI, Shuji, KATOH, Hideki
Format Journal Article
LanguageEnglish
Published Japan Japanese Association for Laboratory Animal Science 2011
Subjects
Animals
Base Sequence
Callithrix - genetics
common marmoset
DNA - analysis
Female
Genes, sry - genetics
Kruppel-Like Transcription Factors - genetics
Male
Molecular Sequence Data
Polymerase Chain Reaction - veterinary
Polymorphism, Restriction Fragment Length
Sex Determination Analysis - methods
sex identification
Y Chromosome - genetics
ZFY/ZFX loci
Online AccessGet full text
ISSN1341-1357
1881-7122
DOI10.1538/expanim.60.417

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Abstract We investigated sex determination via the ZFX and ZFY genes using PCR-RFLP in the common marmoset. We designed a novel primer set to detect ZFX and ZFY. A 483-bp band from the ZFX gene and a 471-bp band from the ZFY gene were amplified. Sequencing data of the products amplified from ZFX and ZFY showed the recognition sites of two restriction enzymes, DdeI and MseI, respectively. After digestion of the products using each enzyme, we found that the band patterns between females and males were different. PCR-based sex identification might provide a tool for further breeding studies and experimental embryological studies using marmosets.
AbstractList We investigated sex determination via the ZFX and ZFY genes using PCR-RFLP in the common marmoset. We designed a novel primer set to detect ZFX and ZFY. A 483-bp band from the ZFX gene and a 471-bp band from the ZFY gene were amplified. Sequencing data of the products amplified from ZFX and ZFY showed the recognition sites of two restriction enzymes, DdeI and MseI, respectively. After digestion of the products using each enzyme, we found that the band patterns between females and males were different. PCR-based sex identification might provide a tool for further breeding studies and experimental embryological studies using marmosets.
Author TAKABAYASHI, Shuji
KATOH, Hideki
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SubjectTerms Animals
Base Sequence
Callithrix - genetics
common marmoset
DNA - analysis
Female
Genes, sry - genetics
Kruppel-Like Transcription Factors - genetics
Male
Molecular Sequence Data
Polymerase Chain Reaction - veterinary
Polymorphism, Restriction Fragment Length
Sex Determination Analysis - methods
sex identification
Y Chromosome - genetics
ZFY/ZFX loci
Title Sex Identification Using the ZFX and ZFY Genes in Common Marmosets (Callithrix jacchus)
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https://www.ncbi.nlm.nih.gov/pubmed/21791881
Volume 60
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