Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells - Lee, Joo-Hyoung; Park, Jong-Ho; Park, Sun-Hye; Kim, Sun-Hong; Kim, Jee Yon; Min, Jeong-Ki; Lee, Gyun Min; Kim, Yeon-Gu (Applied microbiology and biotechnology, 2018)
Keywords: Amplification; Animals; Antigen T (large); Antigens; Antigens, Polyomavirus Transforming - genetics; Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; Cell culture; Cell lines; Chinese hamsters; CHO Cells; Cricetinae; Cricetulus; Deoxyribonucleic acid; Dihydrofolate reductase; DNA; EBNA-1 protein; Epstein-Barr virus; Epstein-Barr Virus Nuclear Antigens - genetics; Fc receptors; Fusion protein; Gene Amplification; Gene expression; Genetic aspects; Glycan; Human gammaherpesvirus 4; Life Sciences; messenger RNA; Methods; Methotrexate; Microbial Genetics and Genomics; Microbiology; Nucleic Acid Amplification Techniques - methods; Physiological aspects; production technology; Protein synthesis; Proteins; R&D; recombinant proteins; Recombinant Proteins - genetics; Reductases; Research & development; research and development; Transfection; Viruses; EBNA-1; Gene amplification; TGE; CHO cells; Fc-fusion protein; PyLT