Efficient genome editing in filamentous fungi via an improved CRISPR‐Cas9 ribonucleoprotein method facilitated by chemical reagents - Zou, Gen; Xiao, Meili; Chai, Shunxing; Zhu, Zhihua; Wang, Ying; Zhou, Zhihua (Microbial biotechnology, 2021)
Keywords: Cell division; Cell membranes; Cordyceps - genetics; CRISPR; CRISPR-Cas Systems; Efficiency; Fungi; Fungi - genetics; Gene Editing; Genes; Genetic transformation; Genomes; gRNA; Hypocreales - genetics; Membrane permeability; Mutagenesis; Mutation; Mycelia; Nuclease; Permeability; Plasmids; Polyethylene glycol; Proteins; Protoplasts; Protoplasts - metabolism; Reagents; Regeneration; Ribonucleoproteins - genetics; Ribonucleoproteins - metabolism; RNA, Guide, CRISPR-Cas Systems; Surfactants