Engineering the oleaginous yeast Yarrowia lipolytica to produce limonene from waste cooking oil

• Published in: Biotechnology for biofuels Vol. 12; no. 1; p. 241
• Main Authors: Pang, Yaru, Zhao, Yakun, Li, Shenglong, Zhao, Yu, Li, Jian, Hu, Zhihui, Zhang, Cuiying, Xiao, Dongguang, Yu, Aiqun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.10.2019; BioMed Central; BMC
• Subjects: Batch culture; batch fermentation; Biological activity; Biosynthesis; By-products; Carbon; Carbon sources; Cell culture; Citrus limon; Cooking; cooking fats and oils; Cooking oils; Cultivation; culture flasks; dietary supplements; Edible vegetable oils; Enzymes; Factories; Fatty acids; Fermentation; Fermentation optimization; Flasks; Functional foods; Functional foods & nutraceuticals; gene overexpression; Genes; Genetic aspects; Genetic engineering; genetically engineered microorganisms; Glucose; Hydroxymethylglutaryl-CoA reductase; Industrial engineering; Industrial production; Kinases; kitchen waste; Limonene; Manufacturing engineering; Mentha spicata; Metabolic engineering; Metabolism; Methods; Mevalonate pathway; Mevalonic acid; Microbial genetic engineering; Microorganisms; Natural products; Oils & fats; Optimization; Pharmaceutical industry; Production processes; Reductases; Waste cooking oil; Yarrowia lipolytica; Yeast; yeasts; Yeasts (Fungi); China; Waste cooking oil; Metabolic engineering; Limonene; Fermentation optimization; Mevalonate pathway; Yarrowia lipolytica

Limonene is an important biologically active natural product widely used in the food, cosmetic, nutraceutical and pharmaceutical industries. However, the low abundance of limonene in plants renders their isolation from plant sources non-economically viable. Therefore, engineering microbes into micro...