SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly

• Published in: Nature structural & molecular biology Vol. 27; no. 3; pp. 260 - 273
• Main Authors: Königs, Vanessa, de Oliveira Freitas Machado, Camila, Arnold, Benjamin, Blümel, Nicole, Solovyeva, Anfisa, Löbbert, Sinah, Schafranek, Michal, Ruiz De Los Mozos, Igor, Wittig, Ilka, McNicoll, Francois, Schulz, Marcel H., Müller-McNicoll, Michaela
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2020; Nature Publishing Group
• Subjects: 631/337/1645/2020; 631/45/612/1230; Amino Acid Sequence; Analysis; Animals; Assembly; Base Sequence; Binding Sites; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Cassettes; Cell Line, Tumor; Cell Nucleus - metabolism; Cell Nucleus - ultrastructure; Decay; Exons; Feedback; Gene expression; Gene Expression Regulation; Homeostasis; Homeostasis - genetics; Introns; Life Sciences; Membrane Biology; Mice; Mouse Embryonic Stem Cells - cytology; Mouse Embryonic Stem Cells - metabolism; Negative feedback; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Nonsense Mediated mRNA Decay; Oligomerization; Open Reading Frames; Poisons; Protein Binding; Protein Biosynthesis; Protein Structure; Protein transport; Proteins; Ribonucleic acid; RNA; RNA Precursors - genetics; RNA Precursors - metabolism; RNA transport; RNA-binding protein; RNA-Binding Proteins - classification; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Serine-Arginine Splicing Factors - genetics; Serine-Arginine Splicing Factors - metabolism; Transcription; Transcription, Genetic; Translation; Europe

SRSF7 is an essential RNA-binding protein whose misexpression promotes cancer. Here, we describe how SRSF7 maintains its protein homeostasis in murine P19 cells using an intricate negative feedback mechanism. SRSF7 binding to its premessenger RNA promotes inclusion of a poison cassette exon and tran...