A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins

• Published in: FEBS letters Vol. 589; no. 19; pp. 2570 - 2577
• Main Authors: Kikhney, Alexey G., Svergun, Dmitri I.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 14.09.2015
• Subjects: Algorithms; Computer Simulation; Disorder; Flexibility; Intrinsically disordered protein; Intrinsically Disordered Proteins - chemistry; Models, Molecular; nuclear magnetic resonance spectroscopy; nucleic acids; Pliability; profits and margins; Protein Conformation; Protein Folding; proteins; quantitative analysis; Scattering, Small Angle; Small angle scattering; Small-angle X-ray scattering; X-radiation; X-ray diffraction; X-Ray Diffraction - instrumentation; X-Ray Diffraction - methods; Small angle scattering; Intrinsically disordered protein; Flexibility; Disorder; Small-angle X-ray scattering

Small-angle X-ray scattering (SAXS) is a biophysical method to study the overall shape and structural transitions of biological macromolecules in solution. SAXS provides low resolution information on the shape, conformation and assembly state of proteins, nucleic acids and various macromolecular com...