Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces

• Published in: Applied and Environmental Microbiology Vol. 70; no. 12; pp. 7220 - 7228
• Main Authors: Matsuki, Takahiro, Watanabe, Koichi, Fujimoto, Junji, Takada, Toshihiko, Tanaka, Ryuichiro
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.12.2004
• Subjects: Adult; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteriological methods and techniques used in bacteriology; Bacteriology; Bacteroides fragilis; Bacteroides fragilis - genetics; Bacteroides fragilis - isolation & purification; Bifidobacterium; Bifidobacterium - growth & development; Bifidobacterium - isolation & purification; Biological and medical sciences; Clostridium - genetics; Clostridium - isolation & purification; Clostridium coccoides; Clostridium leptum; DNA Primers; Feces; Feces - microbiology; Flora; Fundamental and applied biological sciences. Psychology; Genes; Genes, rRNA; Humans; Microbial Ecology; Microbiology; Polymerase Chain Reaction - methods; Prevotella; Prevotella - genetics; Prevotella - isolation & purification; Real time; Ribonucleic acid; RNA; RNA, Ribosomal, 16S - genetics; Species Specificity; Human; Polymerase chain reaction; Targeting; Ribosomal DNA; Primer; 16S-RNA; Bacteria; Feces; Detection; Real time

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