Biobanking human endometrial tissue and blood specimens: standard operating procedure and importance to reproductive biology research and diagnostic development

• Published in: Fertility and sterility Vol. 95; no. 6; pp. 2120 - 2122.e12
• Main Authors: Sheldon, Elizabeth, Vo, Kim Chi, McIntire, Ramsey A., Aghajanova, Lusine, Zelenko, Zara, Irwin, Juan C., Giudice, Linda C.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.2011; Elsevier
• Subjects: biobanking; Biological and medical sciences; Biology - methods; Biology - standards; Biomedical Research - methods; Biomedical Research - standards; biopsy; Biopsy - methods; Biopsy - standards; blood; Blood Specimen Collection - methods; Blood Specimen Collection - standards; California; compliance; Diagnostic Techniques, Obstetrical and Gynecological - standards; DNA; Endometrium; Endometrium - pathology; Endometrium - surgery; Female; gene expression; good laboratory practices; Gynecologic Surgical Procedures - methods; Gynecologic Surgical Procedures - standards; Gynecology. Andrology. Obstetrics; Humans; hysterectomy; Internal Medicine; ischemia; Medical sciences; National Institutes of Health; Obstetrics and Gynecology; patients; Product Labeling - standards; Quality Control; Reference Standards; Reproduction - physiology; researchers; Specimen Handling - methods; Specimen Handling - standards; standard operating procedure; Tissue Banks - standards; women; California; biobanking; Endometrium; standard operating procedure; Human; Tissue; Gynecology; Development; Biology; Diagnosis; Obstetrics; Standards; Blood
• ISSN: 0015-0282; 1556-5653; 1556-5653
• DOI: 10.1016/j.fertnstert.2011.01.164

To develop a standard operating procedure (SOP) for collection, transport, storage of human endometrial tissue and blood samples, subject and specimen annotation, and establishing sample priorities. The SOP synthesizes sound scientific procedures, the literature on ischemia research, sample collection and gene expression profiling, good laboratory practices, and the authors’ experience of workflow and sample quality. The National Institutes of Health, University of California, San Francisco, Human Endometrial Tissue and DNA Bank. Women undergoing endometrial biopsy or hysterectomy for nonmalignant indications. Collecting, processing, storing, distributing endometrial tissue and blood samples under approved institutional review board protocols and written informed consent from participating subjects. Standard operating procedure. The SOP addresses rigorous and consistent subject annotation, specimen processing and characterization, strict regulatory compliance, and a reference for researchers to track collection and storage times that may influence their research. The comprehensive and systematic approach to the procurement of human blood and endometrial tissue in this SOP ensures the high quality, reliability, and scientific usefulness of biospecimens made available to investigators by the National Institutes of Health, University of California, San Francisco, Human Endometrial Tissue and DNA Bank. The detail and perspective in this SOP also provides a blueprint for implementation of similar collection programs at other institutions.