Improving detection of JC virus by ultrafiltration of cerebrospinal fluid before polymerase chain reaction for the diagnosis of progressive multifocal leukoencephalopathy

• Published in: BMC neurology Vol. 19; no. 1; pp. 252 - 9
• Main Authors: Nakamichi, Kazuo, Kawamoto, Michi, Ishii, Junko, Saijo, Masayuki
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25.10.2019; BioMed Central; BMC
• Subjects: Adult; Cerebrospinal fluid; Diagnosis; Diagnostic imaging; DNA; DNA, Viral - cerebrospinal fluid; Female; Genes; Genetic research; Humans; JC virus; JC Virus - isolation & purification; Leukoencephalopathy; Leukoencephalopathy, Progressive Multifocal - cerebrospinal fluid; Leukoencephalopathy, Progressive Multifocal - diagnosis; Magnetic resonance imaging; Male; Middle Aged; Polymerase chain reaction; Polyomavirus; Progressive multifocal leukoencephalopathy; Real-time PCR testing; Real-Time Polymerase Chain Reaction - methods; Risk factors; Ultrafiltration; Ultrafiltration - methods; Real-time PCR testing; Ultrafiltration; Cerebrospinal fluid; JC virus; Progressive multifocal leukoencephalopathy

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disorder caused by JC virus (JCV). Although detecting JCV DNA in the cerebrospinal fluid (CSF) by real-time polymerase chain reaction (PCR) is useful, diagnosis is difficult when JCV concentrations are low. We therefore aimed to low...