A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene

• Published in: Virology (New York, N.Y.) Vol. 497; pp. 157 - 162
• Main Authors: Gadea, Gilles, Bos, Sandra, Krejbich-Trotot, Pascale, Clain, Elodie, Viranaicken, Wildriss, El-Kalamouni, Chaker, Mavingui, Patrick, Desprès, Philippe
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2016
• Subjects: Animals; antiviral properties; Arbovirus; Cercopithecus aethiops; Cloning, Molecular; Emerging disease; Flavivirus; Gene Expression; Gene Order; Genes, Reporter; Genome, Viral; genomics; GFP reporter; Green Fluorescent Proteins - genetics; Infectious Disease; Molecular clones; neutralizing antibodies; nucleotide sequences; Recombinant virus; Recombination, Genetic; reporter genes; RNA; screening; Vero Cells; viral growth; viruses; Zika virus; Zika Virus - genetics; Zika Virus Infection - virology; Recombinant virus; Emerging disease; Zika virus; GFP reporter; Arbovirus; Flavivirus; Molecular clones

Zika virus (ZIKV) infection is a major public health problem with severe human congenital and neurological anomalies. The screening of anti-ZIKV compounds and neutralizing antibodies needs reliable and rapid virus-based assays. Here, we described a convenient method leading to the rapid production o...