Unravelling evolutionary strategies of yeast for improving galactose utilization through integrated systems level analysis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 29; pp. 12179 - 12184
• Main Authors: Hong, Kuk-Ki, Vongsangnak, Wanwipa, Vemuri, Goutham N, Nielsen, Jens
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 19.07.2011; National Acad Sciences
• Subjects: Base Sequence; Bioengineering; Bioengineering - methods; Biofuels; Biological Evolution; Biological Sciences; biosynthesis; cAMP-dependent protein kinase; Carbohydrate metabolism; Carbohydrates; Carbon; Chemicals; Chromatography, High Pressure Liquid; DNA Primers - genetics; Ergosterol; escherichia-coli; Evolution; evolutionary adaptation; Fuel technology; Galactose; Galactose - metabolism; Galactose - pharmacokinetics; Gas Chromatography-Mass Spectrometry; Gene Expression Profiling; Genes; Genetic mutation; Genomes; Genotype & phenotype; glucose; Glycogen; Growth rate; industrial; metabolic engineering; Metabolic Networks and Pathways - genetics; Metabolism; Molecular modelling; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutants; Mutation; network; Phenotype; Phenotypes; phosphoglucomutase; Protein kinase A; proteins; Ras protein; ras Proteins - genetics; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Sequence Analysis, DNA; Species Specificity; specific growth rate; strains; stress tolerance; Sugar; Systems Biology - methods; Yeast; Yeasts

Identification of the underlying molecular mechanisms for a derived phenotype by adaptive evolution is difficult. Here, we performed a systems-level inquiry into the metabolic changes occurring in the yeast Saccharomyces cerevisiae as a result of its adaptive evolution to increase its specific growt...