Automated analysis of high-throughput B-cell sequencing data reveals a high frequency of novel immunoglobulin V gene segment alleles

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 8; pp. E862 - E870
• Main Authors: Gadala-Maria, Daniel, Yaari, Gur, Uduman, Mohamed, Kleinstein, Steven H.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 24.02.2015; National Acad Sciences
• Series: PNAS Plus
• Subjects: adaptive immunity; Alleles; Automation; B-lymphocytes; B-Lymphocytes - metabolism; Base Sequence; Biological Sciences; Cells; Databases, Genetic; Gene Rearrangement, B-Lymphocyte; Genes; Genes, Immunoglobulin; Genotype; Genotype & phenotype; germ cells; high-throughput nucleotide sequencing; High-Throughput Nucleotide Sequencing - methods; Humans; Immunoglobulin Variable Region - genetics; Immunoglobulins; Mutation - genetics; Mutation Rate; PNAS Plus; Polymorphism; Polymorphism, Genetic; receptors; Software; V(D)J Recombination - genetics; somatic hypermutation; next-generation sequencing; adaptive immunity; variable gene segment; B-cell repertoire

Individual variation in germline and expressed B-cell immunoglobulin (Ig) repertoires has been associated with aging, disease susceptibility, and differential response to infection and vaccination. Repertoire properties can now be studied at large-scale through next-generation sequencing of rearrang...