Hotair mediates hepatocarcinogenesis through suppressing miRNA-218 expression and activating P14 and P16 signaling

• Published in: Journal of hepatology Vol. 63; no. 4; pp. 886 - 895
• Main Authors: Fu, Wei-Ming, Zhu, Xiao, Wang, Wei-Mao, Lu, Ying-Fei, Hu, Bao-Guang, Wang, Hua, Liang, Wei-Cheng, Wang, Shan-Shan, Ko, Chun-Hay, Waye, Mary Miu-Yee, Kung, Hsiang-Fu, Li, Gang, Zhang, Jin-Fang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2015
• Subjects: Animals; Blotting, Western; Carcinogenesis - genetics; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Cell Proliferation; Cell Survival; Cyclin-Dependent Kinase Inhibitor p16; Gastroenterology and Hepatology; Gene Expression Regulation, Neoplastic; Hepatocellular carcinoma; Hotair; Humans; Immunoprecipitation; Liver Neoplasms, Experimental - genetics; Liver Neoplasms, Experimental - metabolism; Liver Neoplasms, Experimental - pathology; Mice; microR-218; MicroRNAs - biosynthesis; MicroRNAs - genetics; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; P14; P16; Promoter Regions, Genetic; Proteins - genetics; Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - genetics; RNA, Neoplasm - genetics; Signal Transduction - genetics; lncRNAs; ceRNA; siBmi-1; 3′UTR; Lv-miR218; HCC; Hepatocellular carcinoma; siRNA; MTT; miR-218; microR-218; Hotair; P14; CDS; PRC2; P16; NC; shRNA; Bmi-1; siHotair; RIP; miRNA; PI; EZH2; microRNAs; polycomb repressive complex 2; small interfering RNA; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; specific siRNA of Bmi-1; B lymphoma mouse Moloney leukemia virus insertion region 1; Enhancer of zeste homolog 2; RNA immunoprecipitation; the coding sequence; propidium iodide; specific siRNA of Hotair; hepatocellular carcinoma; negative control RNA duplex; 3′ untranslated region; Long non-coding RNAs; competitive endogenous RNAs; short-hairpin RNA; Hox transcript antisense intergenic RNA; lentiviral pre-miR218 vector; microRNA-218

[Display omitted] Long non-coding RNA Hotair has been considered as a pro-oncogene in multiple cancers. Although there is emerging evidence that reveals its biological function and the association with clinical prognosis, the precise mechanism remains largely elusive. We investigated the function an...