Hotair mediates hepatocarcinogenesis through suppressing miRNA-218 expression and activating P14 and P16 signaling - Fu, Wei-Ming; Zhu, Xiao; Wang, Wei-Mao; Lu, Ying-Fei; Hu, Bao-Guang; Wang, Hua; Liang, Wei-Cheng; Wang, Shan-Shan; Ko, Chun-Hay; Waye, Mary Miu-Yee; Kung, Hsiang-Fu; Li, Gang; et al. (Journal of hepatology, 2015)
Keywords: Animals; Blotting, Western; Carcinogenesis - genetics; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Cell Proliferation; Cell Survival; Cyclin-Dependent Kinase Inhibitor p16; Gastroenterology and Hepatology; Gene Expression Regulation, Neoplastic; Hepatocellular carcinoma; Hotair; Humans; Immunoprecipitation; Liver Neoplasms, Experimental - genetics; Liver Neoplasms, Experimental - metabolism; Liver Neoplasms, Experimental - pathology; Mice; microR-218; MicroRNAs - biosynthesis; MicroRNAs - genetics; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; P14; P16; Promoter Regions, Genetic; Proteins - genetics; Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - genetics; RNA, Neoplasm - genetics; Signal Transduction - genetics; lncRNAs; ceRNA; siBmi-1; 3′UTR; Lv-miR218; HCC; Hepatocellular carcinoma; siRNA; MTT; miR-218; microR-218; Hotair; P14; CDS; PRC2; P16; NC; shRNA; Bmi-1; siHotair; RIP; miRNA; PI; EZH2; microRNAs; polycomb repressive complex 2; small interfering RNA; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; specific siRNA of Bmi-1; B lymphoma mouse Moloney leukemia virus insertion region 1; Enhancer of zeste homolog 2; RNA immunoprecipitation; the coding sequence; propidium iodide; specific siRNA of Hotair; hepatocellular carcinoma; negative control RNA duplex; 3′ untranslated region; Long non-coding RNAs; competitive endogenous RNAs; short-hairpin RNA; Hox transcript antisense intergenic RNA; lentiviral pre-miR218 vector; microRNA-218