Mutation in the putative ketoacyl-ACP reductase CaKR1 induces loss of pungency in Capsicum

• Published in: Theoretical and applied genetics Vol. 132; no. 1; pp. 65 - 80
• Main Authors: Koeda, Sota, Sato, Kosuke, Saito, Hiroki, Nagano, Atsushi J., Yasugi, Masaki, Kudoh, Hiroshi, Tanaka, Yoshiyuki
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2019; Springer; Springer Nature B.V
• Subjects: 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase - genetics; active sites; Acyltransferase; acyltransferases; Agriculture; Amino Acid Sequence; Biochemistry; Biomedical and Life Sciences; Biosynthesis; Biotechnology; branched chain fatty acids; breeding; Capsaicin; Capsaicin - analysis; capsaicinoids; Capsicum; Capsicum - enzymology; Capsicum - genetics; Capsicum chinense; Chromosome 10; Chromosome Mapping; Cloning, Molecular; cultivars; DNA Transposable Elements; Fatty acids; Fatty Acids - analysis; Fatty Acids - chemistry; Fruit - chemistry; Fruit - genetics; fruits; gas chromatography-mass spectrometry; Gene expression; Gene mutation; Gene Silencing; Genes, Plant; Genetic aspects; Genetic Linkage; genomics; hot peppers; Insertion; Introns; Life Sciences; messenger RNA; molecular cloning; Mutation; Original Article; Peppers; Phenotype; Phenotypes; Phylogeny; Physiological aspects; Plant Biochemistry; Plant Breeding; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Pungent principles; recessive genes; Reductase; transaminases; transcription (genetics); Transposons

Key message A putative ketoacyl-ACP reductase ( CaKR1 ) that was not previously known to be associated with pungency of Capsicum was identified from map-based cloning and functional characterization. The pungency of chili pepper fruits is due to the presence of capsaicinoids, which are synthesized t...