灭非灵对尼罗罗非鱼乙酰胆碱酯酶和抗氧化酶活性的影响

为了探究特异性防控药物灭非灵对外来物种尼罗罗非鱼（Oreochromis niloticus）的毒杀机理,测定暴露在亚致死浓度的尼罗罗非鱼脑、鳃、肝脏和肌肉4种组织中乙酰胆碱酯酶（ACh E）和抗氧化酶活性的变化。结果显示：药物暴露之后,ACh E活性在脑、鳃、肝脏和肌肉组织中均受到显著抑制。随着暴露时间的延长,超氧化物歧化酶（SOD）活性在脑和鳃中无显著性变化,而在肝脏和肌肉中显著升高;谷胱甘肽还原酶（GSH）含量在脑中显著下降,在肝脏中显著升高,在鳃和肌肉中先升高后降低;乳酸脱氢酶（LDH）活性在4种组织中均显著升高,且在肝脏中的增幅最大;谷胱甘肽S转移酶（GST）活性在肝脏中显著升高,由...

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Published in广东农业科学 Vol. 42; no. 8; pp. 95 - 100
Main Author 马光明 顾党恩 朱赟杰 牟希东 罗建仁 胡隐昌
Format Journal Article
LanguageChinese
Published 中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 2015
上海海洋大学水产与生命学院,上海201306%中国水产科学研究院珠江水产研究所/农业部热带亚热带水产资源利用与养殖重点实验室,广东广州,510380
Subjects
乙酰胆碱酯酶
尼罗罗非鱼
抗氧化酶
灭非灵
抗氧化酶
尼罗罗非鱼
乙酰胆碱酯酶
灭非灵
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ISSN1004-874X

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Summary:为了探究特异性防控药物灭非灵对外来物种尼罗罗非鱼（Oreochromis niloticus）的毒杀机理,测定暴露在亚致死浓度的尼罗罗非鱼脑、鳃、肝脏和肌肉4种组织中乙酰胆碱酯酶（ACh E）和抗氧化酶活性的变化。结果显示：药物暴露之后,ACh E活性在脑、鳃、肝脏和肌肉组织中均受到显著抑制。随着暴露时间的延长,超氧化物歧化酶（SOD）活性在脑和鳃中无显著性变化,而在肝脏和肌肉中显著升高;谷胱甘肽还原酶（GSH）含量在脑中显著下降,在肝脏中显著升高,在鳃和肌肉中先升高后降低;乳酸脱氢酶（LDH）活性在4种组织中均显著升高,且在肝脏中的增幅最大;谷胱甘肽S转移酶（GST）活性在肝脏中显著升高,由此推测鱼体内可能发生了氧化应激反应。试验结果表明,灭非灵可能是通过抑制尼罗罗非鱼体内ACh E活性,并促使鱼体内氧化应激反应的发生,从而达到其特异性防控的效果。
Bibliography:44-1267/S
Miefeiling; Nile tilapia; acetyl cholinesterase; antioxidant enzyme
In order to explore the toxicity mechanisms of Miefeiling, an effective drug for the Nile tilapia （Oreochromis niloticus ） population control, the activity change of acetylcholinesterase （ACHE） and antioxidant enzyme in Nile tilapia were detected after exposing to Miefeiling with the sublethal concentration. The results showed that, the activity of AChE in the brain, gill, liver and muscle tissues was significantly inhibited after exposing to Miefeiling. With the exposure time increasing, the activity of superoxide dismutase （SOD）had no significant change in brain and gill, but significantly increased in liver and muscle; the content of glutathione reductase （GSH）decreased significantly in brain, but increased in liver, increased in gill and muscle firstly and then decreased. The activity of lactate dehydrogenase （LDH） in the four tissues significantly increased and was the highest in liver. The activity of glutathione s-transferase （
ISSN:1004-874X