RNAi介导的转基因番茄抗TYLCV研究

本研究将TYLCV的AC1和AC2基因中的保守序列嵌合并构建反向重复序列的RNAi载体pBI121-AC1-AC2,通过农杆菌介导法转化番茄获得13株再生植株。结果表明,PCR及RT-PCR检测发现有3株转基因番茄成功表达T-DNA插入的外源基因片段。利用侵染性克隆农杆菌注射及烟粉虱传毒两种接种方法对其进行了抗性鉴定,表明2株转基因番茄植株对TYLCV表现抗性,为抗TYLCV育种提供了新的种质资源。...

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Bibliographic Details
Published in西南农业学报 Vol. 27; no. 6; pp. 2511 - 2516
Main Author 杨玛丽 彭宏 陈天子 张保龙 赵统敏 余文贵 赵丽萍
Format Journal Article
LanguageChinese
Published 江苏省农业科学院蔬菜研究所,江苏南京,210014%江苏省农业科学院农业生物技术研究所,江苏南京,210014 2014
Subjects
RNAi载体
番茄黄化曲叶病毒
转基因番茄
番茄黄化曲叶病毒
RNAi
RNAi载体
TYLCV
Transgenic
转基因番茄
vector
tomato
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2014.06.048

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Summary:本研究将TYLCV的AC1和AC2基因中的保守序列嵌合并构建反向重复序列的RNAi载体pBI121-AC1-AC2,通过农杆菌介导法转化番茄获得13株再生植株。结果表明,PCR及RT-PCR检测发现有3株转基因番茄成功表达T-DNA插入的外源基因片段。利用侵染性克隆农杆菌注射及烟粉虱传毒两种接种方法对其进行了抗性鉴定,表明2株转基因番茄植株对TYLCV表现抗性,为抗TYLCV育种提供了新的种质资源。
Bibliography:51-1213/S
In order to generate engineered resistance to TYLCV,the pBI121-AC1-AC2 RNAi expression vector by using AC1 and AC2 gene of TYLCV was constructed,and thirteen regenerated tomato plants were obtained by agrobacterium-mediated transformation. The results showed that RNA interference sequence of the TYLCV by PCR and RT-PCR identification was introduced into the genome successfully,and three transgenic tomato plants were obtained. Of which,two transgenic plants were confirmed to be resistant to TYLCV significantly,which would provide new germplasm resources for breeding of resistance to TYLCV.
YANG Ma-li,PENG Hong,CHEN Tian-zi,ZHANG Bao-long,ZHAO Tong-min,YU Wen-gui,ZHAO Li-ping（1. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu Nanjing 210014, China; 2. Institute of Agro-Bioteeh- nology, Jiangsu Academy of Agricultural Sciences, Jiangsu Nanjing 210014, China）
TYLCV; RNAi vector; Transgenic tomato
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2014.06.048