Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine

• Published in: Microbial cell factories Vol. 19; no. 1; pp. 129 - 10
• Main Authors: Zhu, Jiangming, Yang, Wei, Wang, Bohua, Liu, Qun, Zhong, Xiaotong, Gao, Quanxiu, Liu, Jiezheng, Huang, Jianzhong, Lin, Baixue, Tao, Yong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 11.06.2020; BioMed Central; BMC
• Subjects: Alanine; Amino acids; Bacillus subtilis - enzymology; Bacterial Proteins - metabolism; Bioavailability; Biosynthesis; Biotechnology; Chemical synthesis; Deactivation; Dipeptides - biosynthesis; E coli; Enzymes; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Glutamate; Glutamate-ammonia ligase; Glutamic acid; Glutamine; Glutamine synthase; Health care; Inactivation; Industrial Microbiology; Industrial production; l-amino acid α-ligase; Ligases; Metabolic Engineering; Microorganisms; Microorganisms, Genetically-Modified - metabolism; Modules; Peptidases; Physiological aspects; Plasmids; Production processes; Proteases; Protein expression; Proteins; Rehabilitation; Substrates; Thermal stability; Transportation systems; Whole-cell biocatalysis; AQ; Metabolic engineering; Glutamine synthase; Whole-cell biocatalysis; L-amino acid α-ligase

L-Alanyl-L-glutamine (AQ) is a functional dipeptide with high water solubility, good thermal stability and high bioavailability. It is widely used in clinical treatment, post-operative rehabilitation, sports health care and other fields. AQ is mainly produced via chemical synthesis which is complica...