Linker histone variant H1t is closely associated with repressed repeat-element chromatin domains in pachytene spermatocytes

• Published in: Epigenetics & chromatin Vol. 13; no. 1; p. 9
• Main Authors: Mahadevan, Iyer Aditya, Kumar, Sanjeev, Rao, Manchanahalli R Satyanarayana
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.03.2020; BioMed Central; BMC
• Subjects: Analysis; Animals; Antibodies; Antigens; Binding sites; Chromatin; Competition; CpG Islands; Deoxyribonucleic acid; DNA; DNA binding proteins; DNA methylation; Enzyme-linked immunosorbent assay; Genes; Genetic aspects; Genomes; Genomics; H1t; Histone Code; Histone variants; Histones; Histones - chemistry; Histones - genetics; Immunoglobulins; LINE; Linker histones; Localization; Long Interspersed Nucleotide Elements; LTR; Male; Mammalian spermatogenesis; Mass spectrometry; Mice; Nucleosomes - chemistry; Nucleosomes - genetics; Occupancy; Pachytene; Pachytene Stage; Proteins; Scientific imaging; Spermatocytes; Spermatocytes - metabolism; Spermatogenesis; Stem cells; Terminal Repeat Sequences; Transposons; Linker histones; H1t; LTR; Mammalian spermatogenesis; Histone variants; LINE

H1t is the major linker histone variant in pachytene spermatocytes, where it constitutes 50-60% of total H1. This linker histone variant was previously reported to localize in the nucleolar rDNA element in mouse spermatocytes. Our main aim was to determine the extra-nucleolar localization of this li...