Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 34; pp. 14234 - 14239
• Main Authors: Ban, Hiroshi, Nishishita, Naoki, Fusaki, Noemi, Tabata, Toshiaki, Saeki, Koichi, Shikamura, Masayuki, Takada, Nozomi, Inoue, Makoto, Hasegawa, Mamoru, Kawamata, Shin, Nishikawa, Shin-Ichi
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 23.08.2011; National Acad Sciences
• Subjects: Animals; Biological Sciences; Biomarkers - metabolism; Blood cells; CD34 antigen; Cell Differentiation; Cord blood; Embryo cells; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Epigenesis, Genetic; Feeder cells; Fetal Blood - cytology; Fibroblasts; Fibroblasts - metabolism; genes; Genetic vectors; Genetic Vectors - genetics; Genomes; Germ Layers - cytology; Germ Layers - metabolism; Hematopoietic stem cells; Humans; Induced pluripotent stem cells; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Inhibitory postsynaptic potentials; Insertion; Mice; Murine respirovirus; Mutation; Pluripotent stem cells; Ribonucleic acid; risk; RNA; RNA viruses; Sendai virus; Sendai virus - genetics; Stem cells; Temperature; Temperature effects; Transgenes - genetics; Vectors; Viruses

After the first report of induced pluripotent stem cells (iPSCs), considerable efforts have been made to develop more efficient methods for generating iPSCs without foreign gene insertions. Here we show that Sendai virus vector, an RNA virus vector that carries no risk of integrating into the host g...