Comparison of targeted metagenomics and IS-Pro methods for analysing the lung microbiome

• Published in: BMC microbiology Vol. 21; no. 1; pp. 228 - 13
• Main Authors: Goolam Mahomed, T, Peters, RPH, Pretorius, GHJ, Goolam Mahomed, A, Ueckermann, V, Kock, MM, Ehlers, MM
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 18.08.2021; BioMed Central; BMC
• Subjects: Abundance; Aged; Aged, 80 and over; Bacteria; Batch processing; Biological diversity; Chronic obstructive pulmonary disease; Composition; Cost analysis; Deoxyribonucleic acid; Diagnosis; DNA; DNA, Bacterial - genetics; Female; Genera; Genomics; Health aspects; Humans; Lung - microbiology; Lung diseases, Obstructive; Lungs; Male; Metagenomics; Metagenomics - methods; Metagenomics - standards; Methods; Microbiomes; Microbiota (Symbiotic organisms); Microbiota - genetics; Microorganisms; Middle Aged; Proteobacteria; Quality control; Relative abundance; RNA sequencing; RNA, Ribosomal, 16S - genetics; rRNA; Software - standards; Species; Sputum; Sputum - microbiology; Statistical analysis; South Africa; England; United Kingdom > UK; United States > US; Cape Town South Africa; Germany

Targeted metagenomics and IS-Pro method are two of the many methods that have been used to study the microbiome. The two methods target different regions of the 16 S rRNA gene. The aim of this study was to compare targeted metagenomics and IS-Pro methods for the ability to discern the microbial comp...