Multiple copies of the oxytetracycline gene cluster in selected Streptomyces rimosus strains can provide significantly increased titers

• Published in: Microbial cell factories Vol. 20; no. 1; pp. 47 - 19
• Main Authors: Pikl, Špela, Carrillo Rincón, Andrés Felipe, Slemc, Lucija, Goranovič, Dušan, Avbelj, Martina, Gjuračić, Krešimir, Sucipto, Hilda, Stare, Katja, Baebler, Špela, Šala, Martin, Guo, Meijin, Luzhetskyy, Andriy, Petković, Hrvoje, Magdevska, Vasilka
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 17.02.2021; BioMed Central; BMC
• Subjects: Bioactive compounds; Biological activity; Biosynthesis; Biosynthetic gene cluster; Chromosomes; Cloning; Deoxyribonucleic acid; DNA; Engineering; Enzymes; Gene expression; Genetic aspects; Industrial microorganisms; Metabolic engineering; Metabolism; Metabolites; Microbiological research; Microbiological synthesis; Microorganisms; Natural products; Oxytetracycline; Physiological aspects; Production processes; Reproducibility; Stability; Streptomyces; Streptomyces rimosus; Yeast; ΦC31; Slovenia; Oxytetracycline; Streptomyces rimosus; ΦC31; Biosynthesis; Biosynthetic gene cluster

Natural products are a valuable source of biologically active compounds that have applications in medicine and agriculture. One disadvantage with natural products is the slow, time-consuming strain improvement regimes that are necessary to ensure sufficient quantities of target compounds for commerc...