An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals

• Published in: Plant methods Vol. 18; no. 1; p. 11
• Main Authors: Xiao, Yongxin, Zhang, Jinying, Li, Yiting, Hsiang, Tom, Zhang, Xingping, Zhu, Yongxing, Du, Xiaoying, Yin, Junliang, Li, Junkai
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 26.01.2022; BioMed Central; BMC
• Subjects: Agricultural chemicals; Agrochemicals; Amino acids; Analysis; Carboxylic acids; Castor beans; Castor oil plant; Cell division; Cell membranes; Fluorescence; Gene expression; Genes; Genetic aspects; Genetic engineering; Genetic transformation; Green fluorescent protein; Histidine; Infiltration; l-Val-PCA (l-valine-phenazine-1-carboxylic acid conjugate); Localization; Lysine; Mathematical models; Membranes; Mesophyll; Methodology; Optimization; Parameters; Pathogens; Pesticides; Phenazine; Phenazine-1-carboxylic acid; Phloem; Physiological aspects; Plasma membranes; Plasma-membrane transporter; Proteins; Recognition; Recognizing and phloem loading; Ricinus; Ricinus communis; Seedlings; Subcellular localization; Translocation; Tubes; Vacuum; Vacuum infiltration; Valine; China; California; United States > US; Germany; L-Val-PCA (L-valine-phenazine-1-carboxylic acid conjugate); Vacuum infiltration; Plasma-membrane transporter; Subcellular localization; Recognizing and phloem loading

Plant plasma membrane transporters play essential roles during the translocation of vectorized agrochemicals. Therefore, transporters associated with phloem loading of vectorized agrochemicals have drawn increasing attention. As a model system, castor bean (Ricinus communis L.) has been widely used...