On-line monitoring of intracellular ATP concentration in Escherichia coli fermentations
A method was developed to provide a real‐time measurement of intracellular adenosine 5′‐triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a cons...
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Published in | Biotechnology and bioengineering Vol. 52; no. 3; pp. 364 - 372 |
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Language | English |
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05.11.1996
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Abstract | A method was developed to provide a real‐time measurement of intracellular adenosine 5′‐triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a construct leads to constant specific activity of firefly luciferase during both the lag phase and exponential growth. When the luciferase substrate, D‐luciferin, is added to the medium, ATP within the cells is utilized in the luciferase‐catalyzed reaction that produces light. The light is carried from the bioreactor to a computer‐based detector by an optical fiber. The detected per cell light emission varies during exponential growth. Analysis of cytoplasm extracts shows that this variance is related to changes in the ATP concentration, which ranges from 1 to 6 times the literature value for KM. Experimental analyses demonstrated that inner filter effects are not a significant factor affecting the use of this system. The method was tested in a benchtop fermentor at cell densities above 13 g/L dry cell weight. A correction factor based on the accumulated light data is calculated and used in real time to account for consumption of luciferin from the culture broth by the light producing reaction. Dissolved oxygen concentrations must be kept above 15% of air saturation to ensure constant light output, but no detectable increase in oxygen demand is seen. The method does not significantly affect growth or production rates. © 1996 John Wiley & Sons, Inc. |
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AbstractList | A method was developed to provide a real‐time measurement of intracellular adenosine 5′‐triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a construct leads to constant specific activity of firefly luciferase during both the lag phase and exponential growth. When the luciferase substrate, D‐luciferin, is added to the medium, ATP within the cells is utilized in the luciferase‐catalyzed reaction that produces light. The light is carried from the bioreactor to a computer‐based detector by an optical fiber. The detected per cell light emission varies during exponential growth. Analysis of cytoplasm extracts shows that this variance is related to changes in the ATP concentration, which ranges from 1 to 6 times the literature value for KM. Experimental analyses demonstrated that inner filter effects are not a significant factor affecting the use of this system. The method was tested in a benchtop fermentor at cell densities above 13 g/L dry cell weight. A correction factor based on the accumulated light data is calculated and used in real time to account for consumption of luciferin from the culture broth by the light producing reaction. Dissolved oxygen concentrations must be kept above 15% of air saturation to ensure constant light output, but no detectable increase in oxygen demand is seen. The method does not significantly affect growth or production rates. © 1996 John Wiley & Sons, Inc. A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a construct leads to constant specific activity of firefly luciferase during both the lag phase and exponential growth. When the luciferase substrate, D-luciferin, is added to the medium, ATP within the cells is utilized in the luciferase-catalyzed reaction that produces light. The light is carried from the bioreactor to a computer-based detector by an optical fiber. The detected per cell light emission varies during exponential growth. Analysis of cytoplasm extracts shows that this variance is related to changes in the ATP concentration, which ranges from 1 to 6 times the literature value for K(M). Experimental analyses demonstrated that inner filter effects are not a significant factor affecting the use of this system. The method was tested in a benchtop fermentor at cell densities above 13 g/L dry cell weight. A correction factor based on the accumulated light data is calculated and used in real time to account for consumption of luciferin from the culture broth by the light producing reaction. Dissolved oxygen concentrations must be kept above 15% of air saturation to ensure constant light output, but no detectable increase in oxygen demand is seen. The method does not significantly affect growth or production rates. (c) 1996 John Wiley & Sons, Inc. A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosphate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a construct leads to constant specific activity of firefly luciferase during both the lag phase and exponential growth. When the luciferase substrate, D-luciferin, is added to the medium, ATP within the cells is utilized in the luciferase-catalyzed reaction that produces light. The light is carried from the bioreactor to a computer-based detector by an optical fiber. The detected per cell light emission varies during exponential growth. Analysis of cytoplasm extracts shows that this variance is related to changes in the ATP concentration, which ranges from 1 to 6 times the literature value for K sub(M). Experimental analyses demonstrated that inner filter effects are not a significant factor affecting the use of this system. The method was tested in a benchtop fermentor at cell densities above 13 g/L dry cell weight. A correction factor based on the accumulated light data is calculated and used in real time to account for consumption of luciferin from the culture broth by the light producing reaction. Dissolved oxygen concentrations must be kept above 15% of air saturation to ensure constant light output, but no detectable increase in oxygen demand is seen. The method does not significantly affect growth or production rates. (DBO) A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The method was tested in a benchtop fermentor at cell densities above 13 g/L dry cell weight. A correction factor based on the accumulated light data is calculated and used in real time to account for consumption of luciferin from the culture broth by the light producing reaction. Dissolved oxygen concentrations must be kept above 15% of air saturation to ensure constant light output, but no detectable increase in oxygen demand is seen. The method does not significantly affect growth or production rates. A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a construct leads to constant specific activity of firefly luciferase during both the lag phase and exponential growth. When the luciferase substrate, D-luciferin, is added to the medium, ATP within the cells is utilized in the luciferase-catalyzed reaction that produces light. The light is carried from the bioreactor to a computer-based detector by an optical fiber. The detected per cell light emission varies during exponential growth. Analysis of cytoplasm extracts shows that this variance is related to changes in the ATP concentration, which ranges from 1 to 6 times the literature value for K(M). Experimental analyses demonstrated that inner filter effects are not a significant factor affecting the use of this system. The method was tested in a benchtop fermentor at cell densities above 13 g/L dry cell weight. A correction factor based on the accumulated light data is calculated and used in real time to account for consumption of luciferin from the culture broth by the light producing reaction. Dissolved oxygen concentrations must be kept above 15% of air saturation to ensure constant light output, but no detectable increase in oxygen demand is seen. The method does not significantly affect growth or production rates. (c) 1996 John Wiley & Sons, Inc. |
Author | Wang, Daniel I. C. Lasko, Daniel R |
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Keywords | Recombinant microorganism Bioluminescence On line Enzyme Escherichia coli Luciferase Method Gene expression Fermentation Reporter gene Bacteria Oxidoreductases Intracellular ATP Monitoring Enterobacteriaceae Optical fiber |
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References | De Wet, J. R., Wood, K. V., Helinski, D. R., DeLuca, M. 1985. Cloning of firefly luciferase cDNA and the expression of active luciferase in Eschericha coli. Proc. Natl. Acad. Sci. USA 82: 7870-7873. Yappert, M. C., Ingle, J. D., Jr. 1989. Correction of polychromatic luminescence signals for inner-filter effects. Appl. Spectrosc. 43: 759-767. Eisinger, J., Flores, J. 1985. Fluorometry of turbid and absorbant samples and the membrane fluidity of intact erythrocytes. Biophys. J. 48: 77-84. Puchalski, M. M., Morra, M. J., von Wandruszka, R. 1991. Assessment of inner filter effect corrections in fluorimetry. Fresenius J. Anal. Chem. 340: 341-344. Brasier, A. R., Tate, J. E., Habener, J. F. 1989. Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines. BioTechniques 7: 1116-1122. Ratzlaff, E. H., Harfmann, R. G., Crouch, S. R. 1984. Absorptioncorrected fiber optic fluorometer. Anal. Chem. 56: 342-347. De Wet, J. R., Wood, K. Y., DeLuca, M., Helinski, D. R., Subramani, S. 1987. Firefly luciferase gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737. Gould, S. J., Subramani, S. 1988. Firefly luciferase as a tool in molecular and cell biology. Anal. Biochem. 175: 5-13. Lee, R. T., Denburg, J. L., McElroy, W. D. 1970. Substrate-binding properties of firefly luciferase: II. A TP-binding site. Arch. Biochem. Biophys. 141: 38-52. Srinivas, S. P., Mutharasan, R. 1987. Inner filter effects and their interferences in the interpretation of culture fluorescence. Biotech. Bioeng. 30: 769-774. Lundin, A., Thore, A. 1975. Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay. Appl. Microbiol. 30: 713-721. Schleif, R. 1989. DNA looping and regulation of the arabinose operon. Harvey Lect. 84: 27-39. Chapman, A. G., Fall, L., Atkinson, D. E. 1971. Adenylate energy charge in Escherichia coli during growth and starvation. J. Bacteriol. 108: 072-1086. Denburg, J. L., Lee, R. T., McElroy, W. D. 1969. Substrate-binding properties of firefly luciferase: I. Luciferin-binding site. Arch. Biochem. Biophys. 134: 381-394. Lasko, D. R., Wang, D. I. C. 1993. In situ fermentation monitoring with recombinant firefly luciferase. Biotech. Bioeng. 42: 30-36. 1989; 84 1991; 340 1970; 141 1987; 30 1989; 43 1989; 7 1971; 108 1993; 42 1984; 56 1987; 7 1987 1985 1988; 175 1975; 30 1985; 82 1985; 48 1969; 134 Ratzlaff (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB14|cit14) 1984; 56 Lee (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB9|cit9) 1970; 141 De Wet (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB4|cit4) 1987; 7 De Wet (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB5|cit5) 1985; 82 Chapman (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB2|cit2) 1971; 108 McElroy (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB12|cit12) 1985 Eisinger (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB6|cit6) 1985; 48 Schleif (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB15|cit15) 1987 Schleif (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB16|cit16) 1989; 84 Denburg (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB3|cit3) 1969; 134 Lundin (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB10|cit10) 1975; 30 Srinivas (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB17|cit17) 1987; 30 Gould (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB7|cit7) 1988; 175 McCapra (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB11|cit11) 1985 Puchalski (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB13|cit13) 1991; 340 Yappert (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB18|cit18) 1989; 43 Brasier (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB1|cit1) 1989; 7 Lasko (10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB8|cit8) 1993; 42 |
References_xml | – volume: 7 start-page: 1116 year: 1989 end-page: 1122 article-title: Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines publication-title: BioTechniques – volume: 82 start-page: 7870 year: 1985 end-page: 7873 article-title: Cloning of firefly luciferase cDNA and the expression of active luciferase in publication-title: Proc. Natl. Acad. Sci. USA – volume: 48 start-page: 77 year: 1985 end-page: 84 article-title: Fluorometry of turbid and absorbant samples and the membrane fluidity of intact erythrocytes publication-title: Biophys. J. – start-page: 359 year: 1985 end-page: 386 – volume: 7 start-page: 725 year: 1987 end-page: 737 article-title: Firefly luciferase gene: Structure and expression in mammalian cells publication-title: Mol. Cell. Biol. – volume: 175 start-page: 5 year: 1988 end-page: 13 article-title: Firefly luciferase as a tool in molecular and cell biology publication-title: Anal. Biochem. – volume: 30 start-page: 769 year: 1987 end-page: 774 article-title: Inner filter effects and their interferences in the interpretation of culture fluorescence publication-title: Biotech. Bioeng. – start-page: 1473 year: 1987 end-page: 1481 – volume: 30 start-page: 713 year: 1975 end-page: 721 article-title: Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay publication-title: Appl. Microbiol. – volume: 340 start-page: 341 year: 1991 end-page: 344 article-title: Assessment of inner filter effect corrections in fluorimetry publication-title: Fresenius J. Anal. Chem. – volume: 43 start-page: 759 year: 1989 end-page: 767 article-title: Correction of polychromatic luminescence signals for inner‐filter effects publication-title: Appl. Spectrosc. – volume: 134 start-page: 381 year: 1969 end-page: 394 article-title: Substrate‐binding properties of firefly luciferase: I. Luciferin‐binding site publication-title: Arch. Biochem. Biophys. – volume: 108 start-page: 072 year: 1971 end-page: 1086 article-title: Adenylate energy charge in during growth and starvation publication-title: J. Bacteriol. – volume: 42 start-page: 30 year: 1993 end-page: 36 article-title: In situ fermentation monitoring with recombinant firefly luciferase publication-title: Biotech. Bioeng. – volume: 84 start-page: 27 year: 1989 end-page: 39 article-title: DNA looping and regulation of the arabinose operon publication-title: Harvey Lect. – volume: 141 start-page: 38 year: 1970 end-page: 52 article-title: Substrate‐binding properties of firefly luciferase: II. A TP‐binding site publication-title: Arch. Biochem. Biophys. – start-page: 387 year: 1985 end-page: 399 – volume: 56 start-page: 342 year: 1984 end-page: 347 article-title: Absorptioncorrected fiber optic fluorometer publication-title: Anal. Chem. – volume: 30 start-page: 713 year: 1975 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB10|cit10 article-title: Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay publication-title: Appl. Microbiol. doi: 10.1128/AM.30.5.713-721.1975 contributor: fullname: Lundin – volume: 175 start-page: 5 year: 1988 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB7|cit7 article-title: Firefly luciferase as a tool in molecular and cell biology publication-title: Anal. Biochem. doi: 10.1016/0003-2697(88)90353-3 contributor: fullname: Gould – volume: 84 start-page: 27 year: 1989 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB16|cit16 article-title: DNA looping and regulation of the arabinose operon publication-title: Harvey Lect. contributor: fullname: Schleif – volume: 7 start-page: 1116 year: 1989 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB1|cit1 article-title: Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines publication-title: BioTechniques contributor: fullname: Brasier – volume: 30 start-page: 769 year: 1987 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB17|cit17 article-title: Inner filter effects and their interferences in the interpretation of culture fluorescence publication-title: Biotech. Bioeng. doi: 10.1002/bit.260300609 contributor: fullname: Srinivas – volume: 82 start-page: 7870 year: 1985 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB5|cit5 article-title: Cloning of firefly luciferase cDNA and the expression of active luciferase in Eschericha coli publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.82.23.7870 contributor: fullname: De Wet – volume: 48 start-page: 77 year: 1985 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB6|cit6 article-title: Fluorometry of turbid and absorbant samples and the membrane fluidity of intact erythrocytes publication-title: Biophys. J. doi: 10.1016/S0006-3495(85)83761-9 contributor: fullname: Eisinger – volume: 141 start-page: 38 year: 1970 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB9|cit9 article-title: Substrate-binding properties of firefly luciferase: II. A TP-binding site publication-title: Arch. Biochem. Biophys. doi: 10.1016/0003-9861(70)90103-7 contributor: fullname: Lee – volume: 42 start-page: 30 year: 1993 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB8|cit8 article-title: In situ fermentation monitoring with recombinant firefly luciferase publication-title: Biotech. Bioeng. doi: 10.1002/bit.260420105 contributor: fullname: Lasko – volume: 108 start-page: 072 year: 1971 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB2|cit2 article-title: Adenylate energy charge in Escherichia coli during growth and starvation publication-title: J. Bacteriol. doi: 10.1128/JB.108.3.1072-1086.1971 contributor: fullname: Chapman – start-page: 359 volume-title: Chemi- and bioluminescence year: 1985 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB11|cit11 contributor: fullname: McCapra – start-page: 1473 volume-title: Escherichia coli and Salmonella typhimurium, cellular and molecular biology year: 1987 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB15|cit15 contributor: fullname: Schleif – volume: 43 start-page: 759 year: 1989 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB18|cit18 article-title: Correction of polychromatic luminescence signals for inner-filter effects publication-title: Appl. Spectrosc. doi: 10.1366/0003702894202120 contributor: fullname: Yappert – volume: 134 start-page: 381 year: 1969 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB3|cit3 article-title: Substrate-binding properties of firefly luciferase: I. Luciferin-binding site publication-title: Arch. Biochem. Biophys. doi: 10.1016/0003-9861(69)90297-5 contributor: fullname: Denburg – volume: 7 start-page: 725 year: 1987 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB4|cit4 article-title: Firefly luciferase gene: Structure and expression in mammalian cells publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.7.2.725 contributor: fullname: De Wet – volume: 340 start-page: 341 year: 1991 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB13|cit13 article-title: Assessment of inner filter effect corrections in fluorimetry publication-title: Fresenius J. Anal. Chem. doi: 10.1007/BF00321578 contributor: fullname: Puchalski – volume: 56 start-page: 342 year: 1984 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB14|cit14 article-title: Absorptioncorrected fiber optic fluorometer publication-title: Anal. Chem. doi: 10.1021/ac00267a009 contributor: fullname: Ratzlaff – start-page: 387 volume-title: Chemi- and Bioluminescence year: 1985 ident: 10.1002/(SICI)1097-0290(19961105)52:3<364::AID-BIT2>3.0.CO;2-I-BIB12|cit12 contributor: fullname: McElroy |
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Snippet | A method was developed to provide a real‐time measurement of intracellular adenosine 5′‐triphosophate (ATP) concentrations in growing Escherichia coli. The... A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The... A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosphate (ATP) concentrations in growing Escherichia coli. The... |
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SubjectTerms | Biological and medical sciences Biotechnology Escherichia coli fiber optic firefly luciferase Fundamental and applied biological sciences. Psychology in situ luminescence Methods. Procedures. Technologies Others Various methods and equipments |
Title | On-line monitoring of intracellular ATP concentration in Escherichia coli fermentations |
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