HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量

目的 建立以HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚含量的方法。方法 色谱柱为SHIADZU-GL Inertsil？ ODS-3 RP C18，流动相为甲醇-水（60：40），检测波长为285 nm，流速为1.0 ml/min，柱温为40℃，外标法定量。结果 醋酸地塞米松、樟脑和苯酚的线性范围分别为4.024~40.24、101.7~2 033和10.38~425.2 μg/ml，相关系数r 〉0.999 5；三者的平均回收率分别为101.2%（RSD为0.56%）、99.89%（RSD为0.72%）、100.2%（RSD为0.97%）；重复性试验中RSD均小于1.5%。结论...

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Bibliographic Details
Published in药学实践杂志 Vol. 35; no. 6; pp. 551 - 553
Main Author 王娅俐;彭贤东;郝伟迤;王丹;郭廷东;唐志立
Format Journal Article
LanguageChinese
Published 南充市中心医院药学部,四川南充,637000 2017
Subjects
乳膏
含量测定
樟脑
苯酚
醋酸地塞米松
高效液相色谱
含量测定
乳膏
dexamethasone acetate
camphor
phenol
高效液相色谱
醋酸地塞米松
cream
determination
苯酚
HPLC
樟脑
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ISSN1006-0111
DOI10.3969/j.issn.1006-0111.2017.06.017

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Summary:目的 建立以HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚含量的方法。方法 色谱柱为SHIADZU-GL Inertsil？ ODS-3 RP C18，流动相为甲醇-水（60：40），检测波长为285 nm，流速为1.0 ml/min，柱温为40℃，外标法定量。结果 醋酸地塞米松、樟脑和苯酚的线性范围分别为4.024~40.24、101.7~2 033和10.38~425.2 μg/ml，相关系数r 〉0.999 5；三者的平均回收率分别为101.2%（RSD为0.56%）、99.89%（RSD为0.72%）、100.2%（RSD为0.97%）；重复性试验中RSD均小于1.5%。结论 本方法简便、准确，重复性好，可用于地米樟酚乳膏的质量控制。
Bibliography:31-1685/R
WANG Yali , PENG Xiandong , HAO Weiyi, WANG Dan , GUO Tingdong , TANG Zhili （Department of Pharmacy ,Nanchong Municipal Central Hospital ,Nanchong ,637000 ,China）
Objective To establish a HPLC method for simultaneously determining the content of dexamethasone acetate, camphor and phenol in compound cream.Methods The separation was performed on a SHIADZU-GL Inertsil ODS-3 RP C18 analytical column with the mobile phase consisting of methanol-water （60：40）. The flow rate was 1.0 ml/min. The detection wave length was 285 nm and the column temperature was 40℃.Results Dexamethasone acetate,camphor and phenol showed good linearity （r〉0.999 5, n=7） within the concentration range of 4.024-40.24,101.7-2 033 and 10.38-425.2 μg/ml,respectively. The average recovery of dexamethasone acetate, camphor and phenol was 101.2%（RSD was 0.56%）,99.89%（RSD was 0.72%）,100.2%（RSD was 0.97%）,respectively. Moreover, the RSDs were less than 1.5% in the repeated tests. Conclusion The method was simple, quick and accurate. It is s
ISSN:1006-0111
DOI:10.3969/j.issn.1006-0111.2017.06.017