PIN1反义核酸对乳腺癌MCF-7细胞增殖及周期的影响

R737.9; 目的 本研究利用PIN1反义核酸阻断乳腺癌MCF-7细胞中PIN1表达,观察其对增殖及周期的影响.方法 构建PIN1反义核酸真核表达质粒pPIN1as,用脂质体转染法将重组质粒转染MCF-7细胞,G418筛选稳定表达重组质粒的克隆,RT-PCR检测PIN1基因表达水平,Western blot检测PIN1蛋白的表达,MTT检测细胞增殖状况,流式细胞术检测细胞周期.结果 稳定表达PIN1反义核酸的MCF-7细胞内PIN1基因表达在mRNA水平和蛋白水平都显著降低;MTT实验显示MCF-7PINas细胞的增殖速度较MCF-7细胞明显减慢(P＜0.05),但FCM显示MCF-7PIN...

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Published in肿瘤防治研究 Vol. 34; no. 12; pp. 917 - 920
Main Authors 周金华, 朱涛, 李红雨, 徐钢, 卢运萍, 马丁
Format Journal Article
LanguageChinese
Published 华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉,430030 2007
Subjects
PIN1
乳腺癌
反义核酸
Online AccessGet full text
ISSN1000-8578
DOI10.3971/j.issn.1000-8578.2007.12.007

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Abstract R737.9; 目的 本研究利用PIN1反义核酸阻断乳腺癌MCF-7细胞中PIN1表达,观察其对增殖及周期的影响.方法 构建PIN1反义核酸真核表达质粒pPIN1as,用脂质体转染法将重组质粒转染MCF-7细胞,G418筛选稳定表达重组质粒的克隆,RT-PCR检测PIN1基因表达水平,Western blot检测PIN1蛋白的表达,MTT检测细胞增殖状况,流式细胞术检测细胞周期.结果 稳定表达PIN1反义核酸的MCF-7细胞内PIN1基因表达在mRNA水平和蛋白水平都显著降低;MTT实验显示MCF-7PINas细胞的增殖速度较MCF-7细胞明显减慢(P＜0.05),但FCM显示MCF-7PINas细胞和MCF-7细胞的周期分布差异无统计学意义(P＞0.05).结论 阻断PIN1可以显著抑制乳腺癌MCF-7细胞的增殖活性,提示PIN1可能成为乳腺癌基因治疗的新的靶点.
AbstractList R737.9; 目的 本研究利用PIN1反义核酸阻断乳腺癌MCF-7细胞中PIN1表达,观察其对增殖及周期的影响.方法 构建PIN1反义核酸真核表达质粒pPIN1as,用脂质体转染法将重组质粒转染MCF-7细胞,G418筛选稳定表达重组质粒的克隆,RT-PCR检测PIN1基因表达水平,Western blot检测PIN1蛋白的表达,MTT检测细胞增殖状况,流式细胞术检测细胞周期.结果 稳定表达PIN1反义核酸的MCF-7细胞内PIN1基因表达在mRNA水平和蛋白水平都显著降低;MTT实验显示MCF-7PINas细胞的增殖速度较MCF-7细胞明显减慢(P＜0.05),但FCM显示MCF-7PINas细胞和MCF-7细胞的周期分布差异无统计学意义(P＞0.05).结论 阻断PIN1可以显著抑制乳腺癌MCF-7细胞的增殖活性,提示PIN1可能成为乳腺癌基因治疗的新的靶点.
Author 周金华
卢运萍
朱涛
马丁
徐钢
李红雨
AuthorAffiliation 华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉,430030
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ZHU Tao
XU Gang
MA Ding
ZHOU Jin-hua
LU Yun-ping
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乳腺癌
反义核酸
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Snippet R737.9; 目的 本研究利用PIN1反义核酸阻断乳腺癌MCF-7细胞中PIN1表达,观察其对增殖及周期的影响.方法 构建PIN1反义核酸真核表达质粒pPIN1as,用脂质体转染法将重组质粒转染MCF-7细胞,G418筛选稳定表达重组质粒的克隆,RT-PCR检测PIN1基因表达水平,Western...
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StartPage 917
Title PIN1反义核酸对乳腺癌MCF-7细胞增殖及周期的影响
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