南瓜AFLP反应体系的建立与优化
以印度南瓜矮生等基因系yd为材料，从叶片中提取基凶组DNA，对酶切、连接、预扩、选扩反应体系及银染方法进行优化研究．建立高效稳定的南瓜AFLP反应体系，即反应体系为DNA模板量500ng、37℃酶切5h、连接3h、预扩产物稀释50倍及Mg^2＋1．5mmoL／L、dNTP0．19mmoL／L的效果最好。...
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| Published in | Guangdong nong ye ke xue no. 2; pp. 15 - 17 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广东省农科院蔬菜研究所,广东省果蔬新技术重点实验室,广东,广州,510640
2009
华南农业大学园艺学院,广东,广州,510640%广东省农科院蔬菜研究所,广东省果蔬新技术重点实验室,广东,广州,510640%华南农业大学园艺学院,广东,广州,510640 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-874X |
| DOI | 10.3969/j.issn.1004-874X.2009.02.007 |
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| Abstract | 以印度南瓜矮生等基因系yd为材料，从叶片中提取基凶组DNA，对酶切、连接、预扩、选扩反应体系及银染方法进行优化研究．建立高效稳定的南瓜AFLP反应体系，即反应体系为DNA模板量500ng、37℃酶切5h、连接3h、预扩产物稀释50倍及Mg^2＋1．5mmoL／L、dNTP0．19mmoL／L的效果最好。 |
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| AbstractList | S642.1; 以印度南瓜矮生等基因系yd为材料,从叶片中提取基因组DNA,对酶切、连接、预扩、选扩反应体系及银染方法进行优化研究,建立高效稳定的南瓜AFLP反应体系,即反应体系为DNA模板量500 ng、37C酶节5 h、连接3 h、预扩产物稀释50倍及Mg2+1.5 mmoL/L、dNTP 0.19 mmoL/L的效果最好. 以印度南瓜矮生等基因系yd为材料，从叶片中提取基凶组DNA，对酶切、连接、预扩、选扩反应体系及银染方法进行优化研究．建立高效稳定的南瓜AFLP反应体系，即反应体系为DNA模板量500ng、37℃酶切5h、连接3h、预扩产物稀释50倍及Mg^2＋1．5mmoL／L、dNTP0．19mmoL／L的效果最好。 |
| Author | 王瑞 黄河勋 于远 陈清华 林毓娥 梁肇均 程志学 |
| AuthorAffiliation | 广东省农科院蔬菜研究所／广东省果蔬新技术重点实验室,广东广州510640 华南农业大学园艺学院,广东广州510640 |
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| Author_FL | CHENG Zhi-xue WANG Rui LIN Yu-e HUANG He-xun CHEN Qing-hua LIANG Zhao-jan YU Yuan |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Establishment and optimization of AFLP reaction system in squash |
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| Publisher | 广东省农科院蔬菜研究所,广东省果蔬新技术重点实验室,广东,广州,510640 华南农业大学园艺学院,广东,广州,510640%广东省农科院蔬菜研究所,广东省果蔬新技术重点实验室,广东,广州,510640%华南农业大学园艺学院,广东,广州,510640 |
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| Snippet | 以印度南瓜矮生等基因系yd为材料，从叶片中提取基凶组DNA，对酶切、连接、预扩、选扩反应体系及银染方法进行优化研究．建立高效稳定的南瓜AFLP反应体系，即反应体系为DNA模板量500ng、37℃酶切5h、连接3h、预扩产物稀释50倍及Mg^2＋1．5mmoL／L、dNTP0．19mmoL／L的效果最好。 S642.1; 以印度南瓜矮生等基因系yd为材料,从叶片中提取基因组DNA,对酶切、连接、预扩、选扩反应体系及银染方法进行优化研究,建立高效稳定的南瓜AFLP反应体系,即反应体系为DNA模板量500 ng、37C酶节5 h、连接3 h、预扩产物稀释50倍及Mg2+1.5 mmoL/L、dNTP 0.19... |
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| SubjectTerms | AFLP 优化 体系 南瓜 |
| Title | 南瓜AFLP反应体系的建立与优化 |
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