Quantitative profiling of the endonuclear glycerophospholipidome of murine embryonic fibroblasts[S]

• Published in: Journal of lipid research Vol. 57; no. 8; pp. 1492 - 1506
• Main Authors: Tribble, Emily K., Ivanova, Pavlina T., Grabon, Aby, Alb, James G., Faenza, Irene, Cocco, Lucio, Brown, H. Alex, Bankaitis, Vytas A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2016; The American Society for Biochemistry and Molecular Biology; Elsevier
• Subjects: Animals; Cell Nucleus - metabolism; Cell Nucleus - ultrastructure; cell signaling; Cells, Cultured; Embryo, Mammalian - chemistry; Fibroblasts - metabolism; Fibroblasts - ultrastructure; lipids; Mice; Nuclear Envelope - metabolism; nuclear receptors/lipid ligands; Phospholipid Transfer Proteins - metabolism; Phospholipids - metabolism; phospholipids/phosphatidylinositol; lipids; phospholipids/phosphatidylinositol; nuclear receptors/lipid ligands; phospholipids/metabolism; cell signaling

A reliable method for purifying envelope-stripped nuclei from immortalized murine embryonic fibroblasts (iMEFs) was established. Quantitative profiling of the glycerophospholipids (GPLs) in envelope-free iMEF nuclei yields several conclusions. First, we find the endonuclear glycerophospholipidome di...