Different Continuous Cropping Spans Significantly Affect Microbial Community Membership and Structure in a Vanilla-Grown Soil as Revealed by Deep Pyrosequencing

• Published in: Microbial ecology Vol. 70; no. 1; pp. 209 - 218
• Main Authors: Xiong, Wu, Zhao, Qingyun, Zhao, Jun, Xun, Weibing, Li, Rong, Zhang, Ruifu, Wu, Huasong, Shen, Qirong
• Format: Journal Article
• Language: English
• Published: New York Springer 01.07.2015; Springer US; Springer Nature B.V
• Subjects: Actinobacteria; Bacillus; Bacteria; bacterial communities; Bacteroidetes; Base Sequence; Basidiomycota; beneficial microorganisms; Biomedical and Life Sciences; Bradyrhizobium; China; Community structure; Computational Biology; Continuous cropping; Correlation; Crop Production - methods; Denaturing Gradient Gel Electrophoresis; DNA Primers - genetics; DNA, Ribosomal Spacer - genetics; Ecology; Firmicutes; fungal communities; Fungi; Fungi - genetics; Fungi - growth & development; Fusarium oxysporum; genes; Geoecology/Natural Processes; internal transcribed spacers; Life Sciences; Microbial activity; Microbial Ecology; Microbiology; Microbiomes; Microbiota; Microorganisms; Molecular Sequence Data; Monoculture; Nature Conservation; Pathogens; ribosomal RNA; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 18S - genetics; rRNA 16S; sequence analysis; Sequence Analysis, DNA; Soil; Soil - chemistry; soil bacteria; soil fungi; SOIL MICROBIOLOGY; Soil microorganisms; Soils; Vanilla; Vanilla - growth & development; vascular wilt; Verrucomicrobia; Verrucomicrobium; Water Quality/Water Pollution; Wilt; Soil bacterial and fungal communities; Continuous cropping; wilt; Vanilla; Pyrosequencing

In the present study, soil bacterial and fungal communities across vanilla continuous cropping time-series fields were assessed through deep pyrosequencing of 16S ribosomal RNA (rRNA) genes and internal transcribed spacer (ITS) regions. The results demonstrated that the long-term monoculture of vani...