Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

• Published in: BMC biotechnology Vol. 17; no. 1; p. 29
• Main Authors: Wu, Jun-Zheng, Liu, Qin, Geng, Xiao-Shan, Li, Kai-Mian, Luo, Li-Juan, Liu, Jin-Ping
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 14.03.2017; BioMed Central
• Subjects: Biofuels; Calories; Cassava; Cellulase; Deoxyribonucleic acid; Digestion; DNA; Efficiency; Enzymes; Gene expression; Gene Expression Profiling - methods; Genetic aspects; Genetic engineering; Genetic Enhancement - methods; Genetic transformation; Genomes; High-Throughput Screening Assays; Incubation; Localization; Manihot - genetics; Manihot esculenta; Mesophyll; Mesophyll Cells - cytology; Methodology; Monosaccharides; Optimization; Physiological aspects; Plant breeding; Plant Proteins - genetics; Plantlets; Plasmids; Polyethylene glycol; Polyethylene Glycols; Protein Engineering - methods; Proteins; Protoplast; Protoplasts; Protoplasts - cytology; Signal transduction; Transfection; Beijing China; China; Japan; Transient expression system; Manihot esculenta; Protoplast isolation; Subcellular localization; Cassava

Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have been used for transgenic breeding and gene function study, a quick, easy and large-scal...