Purification and properties of trehalase from rat intestinal mucosal cells

• Published in: Journal of biochemistry (Tokyo) Vol. 81; no. 4; pp. 1041 - 1049
• Main Authors: Nakano, M, Miyakawa, M. (Aichi Medical Coll., Nagakute (Japan)), Sumi, Y. (Nagoya Univ. (Japan). Faculty of Medicine)
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.1977
• Subjects: 4-Chloromercuribenzenesulfonate - pharmacology; Acetates - pharmacology; Animals; Cations, Divalent - pharmacology; Ethylmaleimide - pharmacology; Hydrogen-Ion Concentration; Intestinal Mucosa - enzymology; Kinetics; Mercury - pharmacology; Molecular Weight; Rats; Sodium Dodecyl Sulfate - pharmacology; Trehalase - isolation & purification; Trehalase - metabolism; Tromethamine - pharmacology

Rat intestinal trehalase was solubilized from microvillous membrane and purified about 30-fold from the membrane fraction. The members fraction was digested with papain and then solubilized with a mixture of 0.5% Triton X-100 and 0.2% sodium deoxycholate. Sohibilized supernatant was precipitated wit...