Characterization of the Cryptic AV3 Promoter of Ageratum Yellow Vein Virus in Prokaryotic and Eukaryotic Systems

• Published in: PloS one Vol. 9; no. 9; p. e108608
• Main Authors: Wang, Wei-Chen, Wu, Chia-Ying, Lai, Yi-Chin, Lin, Na-Sheng, Hsu, Yau-Heiu, Hu, Chung-Chi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 2014; Public Library of Science (PLoS)
• Subjects: Abutilon; Ageratum; Ageratum - virology; Agrobacterium - genetics; Baking yeast; Base Sequence; Begomovirus - genetics; Begomovirus - metabolism; Biological Evolution; Biology and Life Sciences; Biotechnology; Cloning; Coding; Deoxyribonucleic acid; DNA; DNA-binding protein; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - virology; Fluorescence; Gene expression; Genome, Viral; Genomes; Green fluorescent protein; Molecular Sequence Data; Nicotiana - virology; Nucleotides; Open Reading Frames; Plant Diseases - virology; Plasmids; Plastids; Promoter Regions, Genetic; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - virology; Single-stranded DNA; Single-stranded DNA-binding protein; Transcription; Transcription, Genetic; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; Viruses; Yeast; United States > US; Taiwan

A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative -10 and -35 boxes are necessary but not sufficient...