Characterization of the Cryptic AV3 Promoter of Ageratum Yellow Vein Virus in Prokaryotic and Eukaryotic Systems

A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative -10 and -35 boxes are necessary but not sufficient...

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Published inPloS one Vol. 9; no. 9; p. e108608
Main Authors Wang, Wei-Chen, Wu, Chia-Ying, Lai, Yi-Chin, Lin, Na-Sheng, Hsu, Yau-Heiu, Hu, Chung-Chi
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 2014
Public Library of Science (PLoS)
Subjects
Abutilon
Ageratum
Ageratum - virology
Agrobacterium - genetics
Baking yeast
Base Sequence
Begomovirus - genetics
Begomovirus - metabolism
Biological Evolution
Biology and Life Sciences
Biotechnology
Cloning
Coding
Deoxyribonucleic acid
DNA
DNA-binding protein
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - virology
Fluorescence
Gene expression
Genome, Viral
Genomes
Green fluorescent protein
Molecular Sequence Data
Nicotiana - virology
Nucleotides
Open Reading Frames
Plant Diseases - virology
Plasmids
Plastids
Promoter Regions, Genetic
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - virology
Single-stranded DNA
Single-stranded DNA-binding protein
Transcription
Transcription, Genetic
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Viruses
Yeast
United States > US
Taiwan
Online AccessGet full text

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Abstract A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative -10 and -35 boxes are necessary but not sufficient for promoter activity in Escherichia coli, and showed that AYVV-NT AV3 promoter could specifically interact with single-stranded DNA-binding protein and sigma 70 of E. coli involved in transcription. Several AYVV-NT-encoded proteins were found to increase the activity of AV3 promoter. The transcription start sites downstream to AV3 promoter were mapped to nucleotide positions 803 or 805 in E. coli, and 856 in Nicotiana benthamiana. The eukaryotic activity of AV3 promoter and the translatability of a short downstream open reading frame were further confirmed by using a green fluorescent protein reporter construct in yeast (Saccharomyces cerevisiae) cells. These results suggested that AV3 promoter might be a remnant of evolution that retained cryptic activity at present.
AbstractList A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative −10 and −35 boxes are necessary but not sufficient for promoter activity in Escherichia coli, and showed that AYVV-NT AV3 promoter could specifically interact with single-stranded DNA-binding protein and sigma 70 of E. coli involved in transcription. Several AYVV-NT-encoded proteins were found to increase the activity of AV3 promoter. The transcription start sites downstream to AV3 promoter were mapped to nucleotide positions 803 or 805 in E. coli, and 856 in Nicotiana benthamiana. The eukaryotic activity of AV3 promoter and the translatability of a short downstream open reading frame were further confirmed by using a green fluorescent protein reporter construct in yeast (Saccharomyces cerevisiae) cells. These results suggested that AV3 promoter might be a remnant of evolution that retained cryptic activity at present.
A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative -10 and -35 boxes are necessary but not sufficient for promoter activity in Escherichia coli, and showed that AYVV-NT AV3 promoter could specifically interact with single-stranded DNA-binding protein and sigma 70 of E. coli involved in transcription. Several AYVV-NT-encoded proteins were found to increase the activity of AV3 promoter. The transcription start sites downstream to AV3 promoter were mapped to nucleotide positions 803 or 805 in E. coli, and 856 in Nicotiana benthamiana. The eukaryotic activity of AV3 promoter and the translatability of a short downstream open reading frame were further confirmed by using a green fluorescent protein reporter construct in yeast (Saccharomyces cerevisiae) cells. These results suggested that AV3 promoter might be a remnant of evolution that retained cryptic activity at present.A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative -10 and -35 boxes are necessary but not sufficient for promoter activity in Escherichia coli, and showed that AYVV-NT AV3 promoter could specifically interact with single-stranded DNA-binding protein and sigma 70 of E. coli involved in transcription. Several AYVV-NT-encoded proteins were found to increase the activity of AV3 promoter. The transcription start sites downstream to AV3 promoter were mapped to nucleotide positions 803 or 805 in E. coli, and 856 in Nicotiana benthamiana. The eukaryotic activity of AV3 promoter and the translatability of a short downstream open reading frame were further confirmed by using a green fluorescent protein reporter construct in yeast (Saccharomyces cerevisiae) cells. These results suggested that AV3 promoter might be a remnant of evolution that retained cryptic activity at present.
A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative -10 and -35 boxes are necessary but not sufficient for promoter activity in Escherichia coli, and showed that AYVV-NT AV3 promoter could specifically interact with single-stranded DNA-binding protein and sigma 70 of E. coli involved in transcription. Several AYVV-NT-encoded proteins were found to increase the activity of AV3 promoter. The transcription start sites downstream to AV3 promoter were mapped to nucleotide positions 803 or 805 in E. coli, and 856 in Nicotiana benthamiana. The eukaryotic activity of AV3 promoter and the translatability of a short downstream open reading frame were further confirmed by using a green fluorescent protein reporter construct in yeast (Saccharomyces cerevisiae) cells. These results suggested that AV3 promoter might be a remnant of evolution that retained cryptic activity at present.
A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus isolate NT (AYVV-NT). In this study, we demonstrated that the core nucleotides in the putative −10 and −35 boxes are necessary but not sufficient for promoter activity in Escherichia coli , and showed that AYVV-NT AV3 promoter could specifically interact with single-stranded DNA-binding protein and sigma 70 of E. coli involved in transcription. Several AYVV-NT-encoded proteins were found to increase the activity of AV3 promoter. The transcription start sites downstream to AV3 promoter were mapped to nucleotide positions 803 or 805 in E. coli , and 856 in Nicotiana benthamiana . The eukaryotic activity of AV3 promoter and the translatability of a short downstream open reading frame were further confirmed by using a green fluorescent protein reporter construct in yeast ( Saccharomyces cerevisiae ) cells. These results suggested that AV3 promoter might be a remnant of evolution that retained cryptic activity at present.
Author Lai, Yi-Chin
Wang, Wei-Chen
Hsu, Yau-Heiu
Lin, Na-Sheng
Hu, Chung-Chi
Wu, Chia-Ying
AuthorAffiliation 2 Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan
Institute of Infectious Disease and Molecular Medicine, South Africa
1 Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
AuthorAffiliation_xml – name: 2 Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, Taiwan
– name: 1 Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
– name: Institute of Infectious Disease and Molecular Medicine, South Africa
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  givenname: Wei-Chen
  surname: Wang
  fullname: Wang, Wei-Chen
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  givenname: Chia-Ying
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Conceived and designed the experiments: WCW CCH YHH NSL. Performed the experiments: WCW CYW YCL. Analyzed the data: WCW CYW YCL NSL YHH CCH. Contributed reagents/materials/analysis tools: NSL YHH CYW CCH. Wrote the paper: WCW CCH.
Competing Interests: The authors have declared that no competing interests exist.
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Snippet A cryptic prokaryotic promoter, designated AV3 promoter, has been previously identified in certain begomovirus genus, including ageratum yellow vein virus...
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StartPage e108608
SubjectTerms Abutilon
Ageratum
Ageratum - virology
Agrobacterium - genetics
Baking yeast
Base Sequence
Begomovirus - genetics
Begomovirus - metabolism
Biological Evolution
Biology and Life Sciences
Biotechnology
Cloning
Coding
Deoxyribonucleic acid
DNA
DNA-binding protein
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - virology
Fluorescence
Gene expression
Genome, Viral
Genomes
Green fluorescent protein
Molecular Sequence Data
Nicotiana - virology
Nucleotides
Open Reading Frames
Plant Diseases - virology
Plasmids
Plastids
Promoter Regions, Genetic
Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - virology
Single-stranded DNA
Single-stranded DNA-binding protein
Transcription
Transcription, Genetic
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
Viruses
Yeast
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Title Characterization of the Cryptic AV3 Promoter of Ageratum Yellow Vein Virus in Prokaryotic and Eukaryotic Systems
URI https://www.ncbi.nlm.nih.gov/pubmed/25268755
https://www.proquest.com/docview/1979816507
https://www.proquest.com/docview/1567052394
https://pubmed.ncbi.nlm.nih.gov/PMC4182527
https://doaj.org/article/f9207838e1964839be66aa74c15248d8
http://dx.doi.org/10.1371/journal.pone.0108608
Volume 9
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