Glycoproteomics: Past, present and future

• Published in: FEBS letters Vol. 583; no. 11; pp. 1728 - 1735
• Main Authors: Tissot, Bérangère, North, Simon J., Ceroni, Alessio, Pang, Poh-Choo, Panico, Maria, Rosati, Floriana, Capone, Antonietta, Haslam, Stuart M., Dell, Anne, Morris, Howard R.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.06.2009
• Subjects: Carbohydrate sequencing; Carbohydrates - chemistry; conA; concanavalin A; deoxyhexose; dHex; electrospray; Fuc; fucose; Gal; galactose; GalNAc; gas chromatography; Glc; GlcNAc; glucose; Glycodelin; Glycoproteomics; GlycoWorkBench; gp273; Hex; HexNAc; hexose; liquid chromatography; MALDI; Man; mannose; Mass Spectrometry; matrix-assisted laser desorption ionisation; MS/MS; N-acetylgalactosamine; N-acetylglucosamine; N-acetylhexosamine; N-acetylneuraminic acid; NeuAc; NMR; nuclear magnetic resonance; Peptide sequencing; POMC; post-translational modification; pro-opiomelanocortin; Proteomics; PTM; Q-TOF; quadrupole orthogonal acceleration time of flight; tandem mass spectrometry; TIC; time of flight; TOF; total ion chromatogram; Vicia villosa lectin; VVL; PTM; MS/MS; VVL; conA; Peptide sequencing; Fuc; HexNAc; GlcNAc; gp273; GC; GlycoWorkBench; Q-TOF; MALDI; MS; TOF; ES; GalNAc; TIC; POMC; Carbohydrate sequencing; NMR; Glycoproteomics; Gal; LC; dHex; Hex; NeuAc; Man; Mass spectrometry; Glycodelin; Glc

This invited paper reviews the study of protein glycosylation, commonly known as glycoproteomics, beginning with the origins of the subject area in the early 1970s shortly after mass spectrometry was first applied to protein sequencing. We go on to describe current analytical approaches to glycoprot...