IL-10 augments antibody production in in vitro immunized lymphocytes by inducing a Th2-type response and B cell maturation

• Published in: Bioscience, biotechnology, and biochemistry Vol. 68; no. 11; pp. 2279 - 2284
• Main Authors: Xu, Q. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture), Katakura, Y, Yamashita, M, Fang, S, Tamura, T, Matsumoto, S, Aiba, Y, Teruya, K, Osada, K, Nishikawa, R, Shirahata, S
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.11.2004; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Adjuvants, Immunologic; Animals; ANTIBODIES; antibody production; ANTIGENS; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Biological and medical sciences; CD38; Cells, Cultured; CYTOKINES; Cytological Techniques; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Humans; IL-10; Immunity, Cellular - drug effects; IMMUNIZATION; in vitro immunization; Interleukin-10 - pharmacology; LYMPHOCYTES; Magnetics; Mice; Th2 Cells - drug effects; Th2 Cells - immunology; Ripening; Immunization; in vitro immunization; Antibody; Production; CD38; antibody production; In vitro; Lymphocyte; IL-10
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.68.2279

An in vitro immunization (IVI) protocol enables antigen specific antibody production from L-Leucyl-L Leudse methyl ester (LLME)-treated human peripheral blood lymphocytes (PBL) upon antigen stimulation In the presence of IL-2, IL-4, and muramyl dipeptide. In the course of our studies, we have found that IL-10 added at the antigen sensitization significantly augmented antibody production level from the LLME-treated PBL. In the present study, we tried to demonstrate the role of IL-10 In the augmentation of antibody production in an IV[ protocol by clarifying the cytokine expression profiles In CD4+ and CD8+ T cells. The results showed that IL-10 skewed the Th1/Th2 balance to Th2-type responses by suppressing Thl-type cytokine production and augmenting Th2-type cytokine production In CD4+ and CD8+ T cells, as well as in CD19+ B cells Furthermore, IL-10 augmented the expression of CD3g, an antigen marker of plasma cells, on B cells, which clearly Indicates that IL-10 promoted differentiation and maturation of B cells In an WI protocol. These results Indicate that IL-10 plays an important role in setting the cellular milieu to produce antibodies In an IVI protocol.