The Neonatal and Adult Human Testis Defined at the Single-Cell Level

• Published in: Cell reports (Cambridge) Vol. 26; no. 6; pp. 1501 - 1517.e4
• Main Authors: Sohni, Abhishek, Tan, Kun, Song, Hye-Won, Burow, Dana, de Rooij, Dirk G., Laurent, Louise, Hsieh, Tung-Chin, Rabah, Raja, Hammoud, Saher Sue, Vicini, Elena, Wilkinson, Miles F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.02.2019; Elsevier
• Subjects: Adult; Cell Differentiation; Cells, Cultured; germ cells; Humans; Infant, Newborn; Leydig cells; Male; peritubular myoid cells; primordial germ cells; Sertoli cells; Single-Cell Analysis - methods; single-cell RNA sequencing; spermatogenesis; spermatogonia; Spermatogonia - cytology; Spermatogonia - metabolism; spermatogonial stem cells; testes; Testis - cytology; Testis - growth & development; single-cell RNA sequencing; spermatogonia; spermatogonial stem cells; peritubular myoid cells; testes; Leydig cells; spermatogenesis; primordial germ cells; germ cells; Sertoli cells

Spermatogenesis has been intensely studied in rodents but remains poorly understood in humans. Here, we used single-cell RNA sequencing to analyze human testes. Clustering analysis of neonatal testes reveals several cell subsets, including cell populations with characteristics of primordial germ cel...