Production of Recombinant Polypeptides Containing One GA-Module and Analysis of Their Ability to Bind to Human Albumin

• Published in: Bulletin of experimental biology and medicine Vol. 162; no. 1; pp. 66 - 70
• Main Authors: Bormotova, E. A., Gupalova, T. V.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2016; Springer; Springer Nature B.V
• Subjects: Albumin; Amino Acid Sequence; Amino acids; Anaerobic bacteria; Bacterial Proteins - biosynthesis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Base Sequence; Binding Sites; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cloning, Molecular; Escherichia coli - chemistry; Escherichia coli - genetics; Gene Expression; Human serum albumin; Humans; Internal Medicine; Laboratory Medicine; Pathology; Peptostreptococcus magnus; Polypeptides; Protein Binding; Protein G; Protein Interaction Domains and Motifs; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Serum Albumin - chemistry; Solutions; Strains (organisms); Streptococcus; Streptococcus - chemistry; Streptococcus - genetics; GA-module; human serum albumin binding polypeptide; Streptococcus group G
• DOI: 10.1007/s10517-016-3547-3
• ISSN: 0007-4888

Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria Peptostreptococcus magnus contains one HSA-binding GA-module. Protein G from group G and C Streptococcus strains isolated from humans has HSA-binding region consisting of three GA-modules. HSA-binding protein containing two GA-modules was found in strains of group G Streptococcus of animal origin. We obtained two recombinant polypeptides GA1 and GA2 congaing one GA-module each. Recombinant polypeptide with two GA-modules binds HSA with a much higher affinity than polypeptides GA1 and GA2 containing one GA-module. Polypeptide with the second GAmodule more effectively binds HSA than polypeptides with the GA-module.