Production of Recombinant Polypeptides Containing One GA-Module and Analysis of Their Ability to Bind to Human Albumin
Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria Peptostreptococcus magnus contains one HSA-binding GA-module. Protei...
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Published in | Bulletin of experimental biology and medicine Vol. 162; no. 1; pp. 66 - 70 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.11.2016
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria
Peptostreptococcus magnus
contains one HSA-binding GA-module. Protein G from group G and C
Streptococcus
strains isolated from humans has HSA-binding region consisting of three GA-modules. HSA-binding protein containing two GA-modules was found in strains of group G
Streptococcus
of animal origin. We obtained two recombinant polypeptides GA1 and GA2 congaing one GA-module each. Recombinant polypeptide with two GA-modules binds HSA with a much higher affinity than polypeptides GA1 and GA2 containing one GA-module. Polypeptide with the second GAmodule more effectively binds HSA than polypeptides with the GA-module. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-4888 1573-8221 |
DOI: | 10.1007/s10517-016-3547-3 |