Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples

• Published in: Infectious diseases of poverty Vol. 12; no. 1; pp. 71 - 12
• Main Authors: Aula, Oyime P., McManus, Donald P., Jones, Malcolm K., You, Hong, Cai, Pengfei, Gordon, Catherine A.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.08.2023; BioMed Central; BMC
• Subjects: Deoxyribonucleic acid; Diagnostic tests; DNA; DNA dipstick; Eggs; Ethics; Flocculation; Genetic research; Health aspects; Infection; Infections; Loop-mediated isothermal amplification assay; Medical diagnosis; Methods; Mollusks; Parasitic diseases; Public health; Schistosoma japonicum; Schistosomiasis; Tropical diseases; Typhoons; Urine; Philippines; United States > US; DNA dipstick; Schistosomiasis; Schistosoma japonicum; Flocculation; Loop-mediated isothermal amplification assay

Schistosomiasis remains a public health issue and the need for accurate and affordable diagnostics is crucial in the elimination of the disease. While molecular diagnostics are highly effective, they are expensive, with the main costs been associated with DNA extraction. The DNA dipstick is a rapid,...